PMID: 9172618Sep 1, 1996Paper

Risks of diagnostic errors in pathology research of post-abortion herpetic endometritis: limitations of immunohistochemistry in situ hybridization

Annales de pathologie
Z SandoS Remadi

Abstract

Endometrial Herpes-simplex infection is rare. Less than 10 cases have been reported. The impact of herpetic endometritis and abortion is not known, because of the scarcity of clinical and histological data. We present and discuss two cases of abortion with microscopic intranuclear inclusion of biotin, suggestive of herpetic endometritis. These inclusions were positively marked on immunohistochemistry, using anti-herpes antibodies. Similar results were obtained with in situ hybridization using biotin labeled probes (commonly used in some laboratories). But the normal accumulation of biotin in the endometrial cells of pregnant women diminishes the value of such results in gestational material. In fact the interaction between intranuclear endogenous biotin and the avidin-biotin-peroxidase complex may explain the positive controls, performed without anti-herpes antibodies. The biotin labeled probes may also explain the positive results of in situ hybridization. We strongly support the opinion that in pregnant women, the diagnosis of herpetic endometritis needs methodological precautions to avoid pitfall caused by endogenous biotin.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.