Rituximab-mediated depletion of cynomolgus monkey B cells in vitro in different matrices: possible inhibitory effect of IgG

International Immunopharmacology
Yulia Vugmeyster, Kathy Howell

Abstract

The mechanism of rituximab-mediated depletion of nonmalignant CD20+B cells remains to be clarified. In this report, we examine contributions of complement- and cell-dependent killing to the rituximab-mediated depletion of cynomolgus monkey B cells in the in vitro assay. B cell depletion was assessed in whole blood, buffer, autologus plasma (plasma), heat-inactivated plasma (H/I plasma), and cobra venom factor (CVF)-treated plasma matrices in cynomolgus monkey and human samples. Rituximab-mediated B cell depletion in buffer appeared to be greater than that in whole blood or in autologus plasma. Heat inactivation of plasma resulted in the degree of B cell depletion closer to that seen in buffer, whereas CVF treatment of plasma had no effect on B cell depletion. Addition of IgG to the buffer decreased the degree of B cell depletion. The results of these studies imply that (i) plasma components (including complement) are not the mediators of the rituximab-triggered B cell depletion in the in vitro assay, suggesting that cell-mediated mechanisms are likely to be responsible for in vitro killing of normal B cells, and that (ii) some plasma components appear to inhibit rituximab-mediated B cell depletion in the in vitro assay, with Ig...Continue Reading

References

Jun 8, 2001·American Journal of Hematology·L M Aledort
Aug 18, 2001·Anti-cancer Drugs·D G Maloney
Feb 14, 2002·Seminars in Oncology·David G MaloneyAndrea Rose
Mar 7, 2002·International Reviews of Immunology·A SulicaR B Herberman
Apr 17, 2002·Molecular Immunology·Sergei Radaev, Peter Sun
Oct 3, 2002·Leukemia·C KosmasT Papadaki
Mar 22, 2003·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J BoyeA Engert
Mar 26, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Yulia VugmeysterEleanor Canova-Davis
Jun 5, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·James E CroweRichard N Pierson
Jun 26, 2003·Arthritis Research & Therapy·Robert Eisenberg
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·E AjznerL Muszbek
Aug 30, 2003·International Immunopharmacology·Yulia VugmeysterEleanor Canova-Davis
Sep 10, 2003·Seminars in Cancer Biology·Christoph von Schilling
Oct 9, 2003·Annals of the Rheumatic Diseases·T ShawM C Totoritis
Oct 11, 2003·Transplant Immunology·Carsten SchröderRichard N Pierson

❮ Previous
Next ❯

Citations

Jan 24, 2006·Nature Biotechnology·Huijuan LiTillman U Gerngross
Nov 20, 2009·The AAPS Journal·Mohammad TabriziHamza Suria
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Greg A LazarBassil I Dahiyat
Mar 26, 2013·The Journal of Dermatological Treatment·Sharon BaumAviv Barzilai
Aug 8, 2013·Critical Reviews in Biotechnology·Ana Rita CostaJoana Azeredo
Dec 6, 2011·Clinics in Dermatology·Michael KasperkiewiczDetlef Zillikens
Sep 18, 2009·Annals of the New York Academy of Sciences·Enno SchmidtDetlef Zillikens
Jan 13, 2010·MAbs·Naoko Yamane-Ohnuki, Mitsuo Satoh
Apr 24, 2016·Biochimica Et Biophysica Acta·Ngoc Phuong Lan LeMax Crispin
Feb 14, 2008·Clinical Reviews in Allergy & Immunology·Enno SchmidtMatthias Goebeler
Mar 10, 2011·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Emma CraythorneSaman Warnakulasuriya
Oct 8, 2009·Alternatives to Laboratory Animals : ATLA·Jarrod Bailey
May 6, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shigeru IidaMitsuo Satoh
May 24, 2017·Molecular Cancer Therapeutics·Sofia JärnumChristian Kjellman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenocortical Carcinoma

Adrenocortical carcinoma (ACC) is a rare malignancy of the adrenal cortex, associated with a generally dismal prognosis owing to its aggressive behavior. Here are the latest discoveries pertaining to this disease.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.

Antibody-Dependent Cell Cytotoxicity

Antibody-dependent cellular toxicity refers to the lysis of a target cell by a non-sensitized effector cell of the immune system as a result of antibodies binding to the target cell membrane and engaging the Fc receptors on the immune effector cells. Find the latest research on antibody-dependent cellular toxicity here.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis