RIVET-a tool for in vivo analysis of symbiotically relevant gene expression in Sinorhizobium meliloti

Molecular Plant-microbe Interactions : MPMI
Mengsheng Gao, Max Teplitski

Abstract

Despite significant advances in the development of sensitive tools for studying genetics and signal exchange in legume-rhizobium symbioses, many uncertainties remain about the in vivo role of bacterial and plant signals in symbiotic gene regulation. In this study, we adapted TnpR recombinase-based in vivo expression technology (RIVET) to document gene regulation in Sinorhizobium meliloti. The substrate for TnpR, the res1-tet-res1 cassette, is stably inherited when cloned into a neutral site of the S. meliloti genome. Bicistronic promoterless tnpR-beta-glucuronidase (GUS) reporters were constructed to track expression ("resolution") of symbiotically relevant S. meliloti genes during different stages of the interaction. In proof of principle experiments, the resolution of the nodC::tnpR reporter was detected within 4 h of exposure to micromolar levels of the nod operon inducer luteolin and after overnight incubation in the rhizosphere. RIVET demonstrated that cell division gene ftsZ2 was not strongly expressed in the rhizosphere but was activated inside the nodules and on agar surfaces. Rhizosphere expression of the N-acyl homoserine lactone (AHL) synthase sinI::tnpR-GUS reporter was modest in prequorate microcolonies, and then i...Continue Reading

References

Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J T Mulligan, S R Long
Apr 1, 1994·Journal of Bacteriology·W Margolin, S R Long
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·A CamilliJ J Mekalanos
Jun 11, 1999·Molecular Microbiology·V Oke, S R Long
Nov 4, 2000·Molecular Plant-microbe Interactions : MPMI·A C TimmersG Truchet
Jul 11, 2001·Genes & Development·K M WassarmanS Gottesman
Oct 26, 2001·The Journal of Biological Chemistry·Ruth DanielsJan Michiels
Jun 11, 2003·Molecular Plant-microbe Interactions : MPMI·Michael A DjordjevicUNKNOWN Sinorhizobium DNA Sequencing Consortium
Sep 16, 2003·Molecular Plant-microbe Interactions : MPMI·Mengsheng GaoWolfgang D Bauer
Mar 6, 2004·Molecular Plant-microbe Interactions : MPMI·Anke BeckerJacques Batut
Nov 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Melanie J BarnettSharon R Long
Oct 21, 2005·Social Work in Health Care·H David KossmanMaura J Prescher
Nov 18, 2005·Journal of Bacteriology·Mengsheng GaoWolfgang D Bauer
Apr 7, 2006·Applied and Environmental Microbiology·Xue-Song Zhang, Hai-Ping Cheng
Apr 14, 2006·Molecular Plant-microbe Interactions : MPMI·Delphine CapelaClaude Bruand
Mar 16, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Maria Sanchez-ContrerasJ Allan Downie
Jul 17, 2007·Nature Reviews. Microbiology·Kathryn M JonesGraham C Walker

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Citations

Apr 26, 2011·Applied and Environmental Microbiology·Clarice L HarrisonJoel S Griffitts
Apr 28, 2009·Journal of Bacteriology·Nataliya Gurich, Juan E González
Apr 18, 2014·Proceedings. Biological Sciences·Gabriel E DilanjiStephen J Hagen
Feb 7, 2013·Journal of Microbiological Methods·Mauricio J LozanoAntonio Lagares
Oct 8, 2016·Molecular Plant-microbe Interactions : MPMI·Mengsheng GaoMax Teplitski
Nov 25, 2017·Frontiers in Microbiology·Alice CheccucciAlessio Mengoni

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