Rmax: A systematic approach to evaluate instrument sort performance using center stream catch

Methods : a Companion to Methods in Enzymology
Andrew RiddellLola Martinez

Abstract

Sorting performance can be evaluated with regard to Purity, Yield and/or Recovery of the sorted fraction. Purity is a check on the quality of the sample and the sort decisions made by the instrument. Recovery and Yield definitions vary with some authors regarding both as how efficient the instrument is at sorting the target particles from the original sample, others distinguishing Recovery from Yield, where the former is used to describe the accuracy of the instrument's sort count. Yield and Recovery are often neglected, mostly due to difficulties in their measurement. Purity of the sort product is often cited alone but is not sufficient to evaluate sorting performance. All of these three performance metrics require re-sampling of the sorted fraction. But, unlike Purity, calculating Yield and/or Recovery calls for the absolute counting of particles in the sorted fraction, which may not be feasible, particularly when dealing with rare populations and precious samples. In addition, the counting process itself involves large errors. Here we describe a new metric for evaluating instrument sort Recovery, defined as the number of particles sorted relative to the number of original particles to be sorted. This calculation requires onl...Continue Reading

References

Jan 1, 1992·Cytometry·Y A LazebnikV V Zenin
Jan 1, 1991·Cytometry·B G De GroothJ Greve
Apr 29, 2008·Fertility and Sterility·Jackson Kirkman-Brown, Lars Björndahl

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Citations

Mar 1, 2016·Expert Opinion on Drug Discovery·Ioannis K Moutsatsos, Christian N Parker
May 5, 2016·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Olivia Roos Rodrigues, Simon Monard
Dec 14, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Kamila CzechowskaAttila Tárnok
Oct 22, 2019·European Journal of Immunology·Andrea CossarizzaArturo Zychlinsky

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Software Mentioned

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