RMND5 from Xenopus laevis is an E3 ubiquitin-ligase and functions in early embryonic forebrain development

PloS One
Thorsten PfirrmannThomas Hollemann

Abstract

In Saccharomyces cerevisiae the Gid-complex functions as an ubiquitin-ligase complex that regulates the metabolic switch between glycolysis and gluconeogenesis. In higher organisms six conserved Gid proteins form the CTLH protein-complex with unknown function. Here we show that Rmnd5, the Gid2 orthologue from Xenopus laevis, is an ubiquitin-ligase embedded in a high molecular weight complex. Expression of rmnd5 is strongest in neuronal ectoderm, prospective brain, eyes and ciliated cells of the skin and its suppression results in malformations of the fore- and midbrain. We therefore suggest that Xenopus laevis Rmnd5, as a subunit of the CTLH complex, is a ubiquitin-ligase targeting an unknown factor for polyubiquitination and subsequent proteasomal degradation for proper fore- and midbrain development.

References

Jan 1, 1997·Journal of Neurobiology·N Hirsch, W A Harris
Aug 31, 2000·Journal of Molecular Biology·C NotredameJ Heringa
Aug 9, 2005·Protein Expression and Purification·Tauseef R ButtMichael R Mattern
May 4, 2007·BMC Neuroscience·Nadia TagnaoutiMatthias Kneussel
Jan 25, 2008·The Biochemical Journal·Takeyuki SuzukiYoshiaki Ishigatsubo
Jan 26, 2008·The Journal of Biological Chemistry·Claes AndréassonBernd Bukau
Feb 21, 2008·The Journal of Clinical Endocrinology and Metabolism·Daniel KelbermanMehul T Dattani
Apr 22, 2008·Nucleic Acids Research·A DereeperO Gascuel
Dec 25, 2008·Methods in Molecular Biology·Tadas PanavasTauseef R Butt
Jun 6, 2009·Annual Review of Biochemistry·Raymond J Deshaies, Claudio A P Joazeiro
Aug 20, 2009·Molecular Systems Biology·Sjoerd J L van WijkH Th Marc Timmers
Dec 18, 2009·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Angie Rizzino
Mar 13, 2010·The Open Biochemistry Journal·Koji TomaruYoshiaki Ishigatsubo
May 18, 2010·Algorithms for Molecular Biology : AMB·Gordon BlackshieldsDesmond G Higgins
Jun 12, 2012·Journal of Neurosurgery. Pediatrics·Timothy W VogelAlan R Cohen
Jun 12, 2013·PloS One·Thorsten PfirrmannThomas Hollemann
Sep 24, 2013·Biochimica Et Biophysica Acta·Thomas Sommer, Dieter H Wolf

❮ Previous
Next ❯

Citations

Aug 24, 2018·FEBS Letters·Ruth MenssenDieter H Wolf
Mar 21, 2019·Biological Chemistry·Huaize Liu, Thorsten Pfirrmann
Jul 10, 2019·Scientific Reports·Matthew E R MaitlandCaroline Schild-Poulter
Jul 1, 2017·Open Biology·Louisa M SalemiCaroline Schild-Poulter
Dec 7, 2016·Bioscience Reports·Ore FrancisJosephine C Adams
Jan 28, 2017·Science·Shun-Jia ChenAlexander Varshavsky
Jul 3, 2017·BMC Cancer·Louisa M SalemiCaroline Schild-Poulter
Jul 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Artem MelnykovAlexander Varshavsky
Jun 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Cheng DongJinrong Min
Apr 22, 2016·FEBS Letters·Toshiyasu GotoHiroshi Shibuya
Jan 3, 2020·Biochemistry·Shun-Jia ChenAlexander Varshavsky
Oct 15, 2021·EMBO Reports·Weaam I MohamedMatthias Peter

❮ Previous
Next ❯

Methods Mentioned

BETA
CTLH
in vitro transcription
ubiquitination

Software Mentioned

Excel
TotalLab Quant
Coffee
fr
Phylogeny
SOMA

Related Concepts

Related Feeds

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

© 2021 Meta ULC. All rights reserved