RNA and protein synthesis requirements for the resumption of meiosis in rabbit oocytes: the role of cumulus cells.

Reproduction, Nutrition, Development
J MotlíkJ Fulka

Abstract

In vitro maturation of rabbit cumulus-enclosed oocytes was fully inhibited in alpha-amanitin- (100 micrograms/ml) and cycloheximide- (5 micrograms/ml) supplemented media. The inhibition was reversible and substantially reduced by delaying the addition of alpha-amanitin (2h) or cycloheximide (3 h). In contrast, both drugs did not inhibit germinal vesicle breakdown in denuded oocytes. Co-culture of granulosa cells (1 x 10(6)/ml) with denuded oocytes did not substitute for an intact cumulus. The data presented here suggest that the resumption of meiosis in rabbit cumulus-enclosed oocytes is dependent upon early transcriptional and translational events which probably occur within the cumulus cells.

Citations

Aug 27, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Atef Ali, Marc-André Sirard
Aug 21, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Mourad AssidiMarc-André Sirard
Feb 1, 1994·Molecular Reproduction and Development·L JelínkováP Guerrier
Nov 1, 1990·Molecular Reproduction and Development·J Motlík, Z Rimkevicová
Mar 30, 2007·Zygote : the Biology of Gametes and Early Embryos·G Sánchez ToranzoM I Bühler
Mar 1, 1997·Molecular Reproduction and Development·A G ByskovX Guoliang
Feb 1, 1994·Microscopy Research and Technique·D F Albertini, V Rider
Dec 1, 1990·Molecular Reproduction and Development·J Motlík, M Kubelka
Aug 5, 2016·Genesis : the Journal of Genetics and Development·Reza K OqaniDong Il Jin
Nov 13, 2009·Molecular Reproduction and Development·Hirohisa KyogokuTakashi Miyano
May 5, 2011·Molecular Reproduction and Development·Hirohisa KyogokuJosef Fulka

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