PMID: 8604334Mar 15, 1996Paper

RNA aptamers that bind L-arginine with sub-micromolar dissociation constants and high enantioselectivity

Nucleic Acids Research
A GeigerM Famulok

Abstract

A completely randomized RNA pool as well as a degenerate pool comprised of an RNA sequence which binds citrulline with a dissociation constant of 0 muM were used to select for tight binding arginine specific RNA aptamers. A modified in vitro selection scheme, based on affinity chromatography was applied to allow the enrichment of high affinity solution binders. The selection scheme included a negative selection with the non-cognate ligand citrulline, and a heat denaturation step prior to affinity elution with an excess of the cognate ligand arginine. After 20 cycles the majority of the pools bound specifically to the arginine matrix even after denaturation/renaturation in the presence of 20 mM of a non-cognate amino acid. When denatured and eluted in the presence of 20 mM arginine, the selected RNAs quantitatively washed off the column. These RNA aptamers were cloned and sequenced. Equilibrium dialysis performed with the most abundant clone among the selected sequence revealed Kd values of 330 nM for the RNA/arginine affinity, which is nearly a 200-fold improvement over the tightest binding arginine binding RNAs known to date. Arginine recognition by this RNA is highly enantioselectice: L- arginine is bound 12 000-fold better t...Continue Reading

Citations

May 15, 2002·Electrophoresis·Stacey L Clark, Vincent T Remcho
Jun 22, 2007·Analytical and Bioanalytical Chemistry·Teresa MairalCiara K O'Sullivan
Feb 22, 2012·Analytical and Bioanalytical Chemistry·Ilaria Palchetti, Marco Mascini
Jul 7, 2005·Journal of Molecular Evolution·Irene MajerfeldMichael Yarus
Oct 2, 2009·Journal of Molecular Evolution·Michael YarusRob Knight
Nov 12, 2005·Applied Microbiology and Biotechnology·Daniela ProskeAnsgar Resch
May 27, 2009·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Robert W Griffith
Aug 21, 2013·Biosensors & Bioelectronics·Subash C B GopinathKoichi Awazu
Sep 30, 1998·Chemistry & Biology·R D Knight, L F Landweber
Apr 29, 2000·RNA·R D Knight, L F Landweber
Apr 29, 2000·RNA·A D EllingtonC A Shaw
Mar 24, 2009·Chemical Reviews·Juewen LiuYi Lu
Sep 9, 2011·ACS Nano·Jolene L LauM G Finn
Sep 1, 1996·Nature Biotechnology·A NolteJ P Fürste
Jun 12, 2010·Nature Protocols·Kwame SefahWeihong Tan
Aug 1, 1997·Nature Structural Biology·H A Heus
Dec 1, 1996·Nature Structural Biology·C H Lin, D J Patel
Sep 29, 2011·Lab on a Chip·Meng Jing, Michael T Bowser
Mar 19, 2011·Oligonucleotides·Minjoung JoSoyoun Kim
Sep 29, 2004·Nucleic Acids Research·Laure WeillBruno Sargueil
Jul 23, 1999·Annals of the New York Academy of Sciences·R D Knight, L F Landweber
Apr 20, 2012·Journal of Virology·Subash C B GopinathPenmetcha K R Kumar
Jun 29, 2000·Annual Review of Biochemistry·D S Wilson, J W Szostak
Jun 15, 2005·Annual Review of Biochemistry·Michael YarusRob Knight
May 18, 2007·Annual Review of Biochemistry·S Jarrett Wrenn, Pehr B Harbury
Mar 3, 2010·RNA·Teresa JanasMichael Yarus
Oct 17, 2003·RNA·Catherine LozuponeMichael Yarus
Dec 21, 2010·PloS One·Kwame SefahWeihong Tan
Apr 25, 2012·PloS One·Pedro NadalCiara K O'Sullivan
Jan 1, 2014·PloS One·Kylan SzetoHarold G Craighead
Jan 31, 2007·Biological Chemistry·Piotr BorsukPiotr Weglenski
Jan 25, 2012·International Journal of Molecular Sciences·Guillermo Aquino-Jarquin, Julia D Toscano-Garibay
Mar 23, 2012·Sensors·Subash C B GopinathMakoto Fujimaki
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·S J KlugM Famulok
Aug 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jonathan H Davis, Jack W Szostak
Nov 15, 2012·Journal of Nucleic Acids·Maureen McKeague, Maria C Derosa
Jun 5, 2014·Annual Review of Analytical Chemistry·Xiangling XiongWeihong Tan
Jun 1, 2014·Journal of Pharmaceutical and Biomedical Analysis·Zsuzsanna SzeitnerTamás Mészáros
May 19, 2010·Analytical and Bioanalytical Chemistry·Ying PuWeihong Tan

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