PMID: 7537369Mar 25, 1995Paper

RNA-binding activity of the matK protein encoded by the chloroplast trnK intron from mustard (Sinapis alba L.)

Nucleic Acids Research
K Liere, G Link

Abstract

The chloroplast trnK gene for tRNALys(UUU) from mustard contains a 2574 bp group II intron with a long open reading frame for 524 amino acids. The encoded polypeptide appears to be structurally related to mitochondrial maturases which are involved in splicing. To study the properties of the intron encoded protein, we overexpressed the trnK ORF as a beta-galactosidase fusion protein in E. coli and carried out RNA-protein binding experiments with crude bacterial extracts and the purified fusion protein. Both gel-shift and UV-crosslinking experiments revealed preferential binding to the trnK precursor transcript. Of two other RNA probes containing chloroplast group II introns, the trnG precursor was recognized by the trnK ORF protein, but the rps16 precursor was not. Competition binding experiments indicate that G-residues seem to play a role in RNA-protein interaction. RNA-binding activity of the trnK intron encoded polypeptide is consistent with its suggested function as a plastid maturase, hence justifying the assignment matK for this gene.

References

Jan 1, 1992·Annual Review of Cell Biology·J D Rochaix
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K H WolfeJ D Palmer
Aug 14, 1992·Science·J A Steitz
Sep 1, 1990·Molecular & General Genetics : MGG·E Bergantino, G Carignani
Nov 1, 1991·Science·P A Sharp
Sep 1, 1990·Trends in Biochemical Sciences·A Jacquier
Sep 1, 1990·Molecular & General Genetics : MGG·M Goldschmidt-ClermontJ D Rochaix
Nov 1, 1990·Trends in Biochemical Sciences·A M Lambowitz, P S Perlman
Dec 11, 1989·Nucleic Acids Research·J Nickelsen, G Link
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Aug 25, 1981·Nucleic Acids Research·U RütherB Müller-Hill
Jun 17, 1994·Cell·P A Sharp
Jul 22, 1993·Nature·J L Ferat, F Michel
Feb 1, 1994·Current Genetics·P du JardinB Wathelet
Dec 1, 1993·Trends in Biochemical Sciences·D W Copertino, R B Hallick
Apr 1, 1993·The Plant Journal : for Cell and Molecular Biology·J Nickelsen, G Link
Jul 25, 1993·Nucleic Acids Research·R B HallickE Stutz
Jan 1, 1993·Annual Review of Biochemistry·A M Lambowitz, M Belfort
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Z Svab, P Maliga
Jan 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R SaldanhaA M Lambowitz

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Citations

Jan 1, 1996·Plant Molecular Biology·T HübschmannT Börner
Oct 1, 1996·Plant Molecular Biology·M Sugita, M Sugiura
Jul 11, 2006·Journal of Molecular Evolution·Sabine KahlauRalph Bock
Oct 27, 2010·Plant Molecular Biology·Paul G WolfKathleen M Pryer
Mar 23, 2011·Plant Molecular Biology·Susann WickeDietmar Quandt
Sep 1, 2012·Journal of Experimental Botany·Yin An YaoGang Xu
Jan 13, 2006·Nucleic Acids Research·Livia MerendinoMichel Goldschmidt-Clermont
Mar 24, 2004·RNA·Karl PerronJean-David Rochaix
Jun 23, 2009·PloS One·Joel R McNealClaude W dePamphilis
Mar 3, 2010·Annual Review of Plant Biology·David B SternMaureen R Hanson
Nov 5, 2011·Journal of Plant Physiology·Claudius Marondedze, Ludivine A Thomas
Mar 9, 2010·New Biotechnology·Michael Tillich, Kirsten Krause
Nov 15, 2007·Biochimie·Eva M del Campo, Leonardo M Casano
Oct 1, 2011·The Plant Journal : for Cell and Molecular Biology·Stephanie GlanzUlrich Kück
Jan 28, 2015·Biochimica Et Biophysica Acta·Christian Schmitz-LinneweberOren Ostersetzer-Biran
Nov 18, 2014·Frontiers in Plant Science·Yvonne SchröterThomas Pfannschmidt
Jul 19, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jiantao ZhaoJing Zhang
Dec 1, 2003·American Journal of Botany·Khidir W HiluLars W Chatrou
Apr 1, 2003·American Journal of Botany·Erin R SandersRichard M McCourt
Aug 1, 2007·American Journal of Botany·Michelle M Barthet, Khidir W Hilu
Oct 20, 2009·Gene Regulation and Systems Biology·Eva M del Campo
Oct 20, 2018·Frontiers in Plant Science·Yujiao QuChristian Schmitz-Linneweber
Mar 19, 2020·Plant Direct·Michelle M BarthetEmilie-Katherine Tavernier
Mar 9, 2021·The Plant Journal : for Cell and Molecular Biology·Sofia Shevtsov-TalOren Ostersetzer-Biran
Nov 1, 2013·Plant Physiology·Stefanie HertelChristian Schmitz-Linneweber

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