RNA-binding motif protein 5 inhibits the proliferation of cigarette smoke-transformed BEAS-2B cells through cell cycle arrest and apoptosis

Oncology Reports
Xue-Jiao LvJie Zhang

Abstract

Cigarette smoking has been shown to be the most significant risk factor for lung cancer. Recent studies have also indicated that RNA-binding motif protein 5 (RBM5) can modulate apoptosis and suppress tumor growth. The present study focused on the role of RBM5 in the regulation of cigarette smoke extract (CSE)-induced transformation of bronchial epithelial cells into the cancerous phenotype and its mechanism of action. Herein, we exposed normal BEAS-2B cells for 8 days to varying concentrations of CSE or dimethylsulfoxide (DMSO), followed by a recovery period of 2 weeks. Next, the RBM5 protein was overexpressed in these transformed BEAS-2B cells though lentiviral infection. Later, the morphological changes, cell proliferation, cell cycle, apoptosis, invasion and migration were assessed. In addition, we analyzed the role of RBM5 in xenograft growth. The expression of RBM5 along with the genes related to cell cycle regulation, apoptosis and invasion were also examined. Finally, our results revealed that BEAS-2B cells exposed to 100 µg/ml CSE acquired phenotypic changes and formed tumors in nude mice, indicative of their cancerous transformation and had reduced RBM5 expression. Subsequent overexpression of RBM5 in these cells signi...Continue Reading

References

Jun 23, 2001·Oncogene·L C SutherlandB A Miller
Sep 11, 2002·Apoptosis : an International Journal on Programmed Cell Death·M Mourtada-MaarabouniG T Williams
Sep 10, 2002·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·D Bradley WellingLong-Sheng Chang
Feb 13, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Mirna Mourtada-MaarabouniGwyn T Williams
Jun 20, 2003·Cell Proliferation·Katrien VermeulenZwi N Berneman
Jan 30, 2004·International Journal of Cancer. Journal International Du Cancer·Ylva HedbergGöran Landberg
Jun 12, 2004·Apoptosis : an International Journal on Programmed Cell Death·N D Rintala-Maki, L C Sutherland
Sep 1, 2004·Cell Biochemistry and Function·Nina D Rintala-MakiLeslie C Sutherland
Nov 19, 2004·Cancer Research·Weiguang WangElaina Collie-Duguid
Nov 24, 2004·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·A J SascoK Straif
Apr 21, 2005·Seminars in Cell & Developmental Biology·Tsz Kan Fung, Randy Y C Poon
Oct 8, 2005·Angiogenesis·S RamakrishnanM Geller
Mar 21, 2006·Experimental Cell Research·Mirna Mourtada-MaarabouniGwyn T Williams
Nov 30, 2006·Journal of Cellular Biochemistry·Nina D Rintala-MakiLeslie C Sutherland
Dec 22, 2006·PloS One·Hassan Lemjabbar-AlaouiCarol Basbaum
Jan 2, 2007·TheScientificWorldJournal·Mirna M Maarabouni, Gwyn T Williams
Sep 25, 2008·Nature Reviews. Molecular Cell Biology·Helfrid HocheggerTim Hunt
Oct 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kazuo FushimiJane Y Wu
Feb 27, 2010·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Leslie C SutherlandAndrew G Robinson
Mar 24, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Jonathan Sleeman, Patricia S Steeg
Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Munekazu YamakuchiCharles J Lowenstein
Mar 24, 2010·International Journal of Cancer. Journal International Du Cancer·Takahiko KobayashiRainer K Brachmann
Mar 27, 2010·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Juliana J OhDennis J Slamon
Apr 28, 2012·Journal of Experimental & Clinical Cancer Research : CR·Hong LiangKe Wang
Jul 5, 2012·Journal of Toxicology and Environmental Health. Part a·Houbing DuJianxiang Li
Dec 24, 2013·American Journal of Respiratory Cell and Molecular Biology·Andrea C SchambergerOliver Eickelberg
Sep 17, 2014·World Journal of Biological Chemistry·Sugreev VermaSnehasikta Swarnakar

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