Abstract
The present study aimed to study the roles and underlying mechanisms of human antigen R (HuR) in osteosarcoma (OS) cell progression. It was determined that the HuR mRNA and protein levels were significantly upregulated in OS tissues, compared with that in normal adjacent tissues. HuR expression was negatively associated with miR-142-3p expression, but positively with High Mobility Group AT-Hook 1 (HMGA1). Additionally, knockdown of HuR inhibited OS cells viability, epithelial-mesenchymal transition and promoted cell apoptosis. HuR was determined to harbor binding sites on HMGA1, directly binding to HMGA1, increasing HMGA1 mRNA stability and expression. Notably, the promotion of HuR on HMGA1 expression was attenuated via miR-142-3p overexpression, and miR-142-3p could directly bind to HMGA1 3'untranslated region (UTR). Furthermore, HMGA1 3'UTR with a mutated miR-142-3p binding site did not respond to HuR alterations. Finally, the inhibition of HuR knockdown was attenuated or even reversed via HMGA1 overexpression; therefore, the results of the present study indicated that RNA binding protein HuR may facilitate OS cell progression via competitively binding to HMGA1 with miR-142-3p.
References
Feb 13, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S WeedenUNKNOWN European Osteosarcoma Intergroup
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 25, 2007·Orphanet Journal of Rare Diseases·Piero Picci
May 8, 2009·Current Opinion in Pediatrics·Travis HeareShelley Dell'Orfano
Nov 28, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ling FangBurton B Yang
May 8, 2014·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Guoxing XuYifan Kang
Oct 25, 2014·Cell Death and Differentiation·Y KuwanoK Rokutan
May 20, 2015·FEBS Letters·Nico SteinmeyerWolfgang Eberhardt
Sep 17, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·George R NahasPranela Rameshwar
Oct 24, 2015·Cancer Gene Therapy·R MuralidharanR Ramesh
Apr 8, 2016·Molecular Cancer Research : MCR·Carmella RomeoJonathan R Brody
Feb 14, 2017·BioMed Research International·Feng ChengShuzheng Chen
Mar 28, 2017·Cell Cycle·Ke LiuWenzhao Li
Mar 31, 2017·International Journal of Oncology·Qingqiang YangJianjun He
Apr 11, 2017·International Journal of Oncology·Jing ZhongRen-Xian Cao
Jul 29, 2017·ELife·Ning DaiJoseph Avruch
Aug 5, 2017·Cell Death and Differentiation·Andrea ConteGiovanna Maria Pierantoni
Aug 19, 2017·Journal of Cell Science·Jinhang HuLufeng Zheng