RNA Binding Proteins and Gene Expression Regulation in Trypanosoma cruzi.

Frontiers in Cellular and Infection Microbiology
Bruno A A RomagnoliSamuel Goldenberg

Abstract

The regulation of gene expression in trypanosomatids occurs mainly at the post-transcriptional level. In the case of Trypanosoma cruzi, the characterization of messenger ribonucleoprotein (mRNP) particles has allowed the identification of several classes of RNA binding proteins (RBPs), as well as non-canonical RBPs, associated with mRNA molecules. The protein composition of the mRNPs as well as the localization and functionality of the mRNAs depend on their associated proteins. mRNPs can also be organized into larger complexes forming RNA granules, which function as stress granules or P-bodies depending on the associated proteins. The fate of mRNAs in the cell, and consequently the genes expressed, depends on the set of proteins associated with the messenger molecule. These proteins allow the coordinated expression of mRNAs encoding proteins that are related in function, resulting in the formation of post-transcriptional operons. However, the puzzle posed by the combinatorial association of sets of RBPs with mRNAs and how this relates to the expressed genes remain to be elucidated. One important tool in this endeavor is the use of the CRISPR/CAS system to delete genes encoding RBPs, allowing the evaluation of their effect on th...Continue Reading

References

Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K L PerryN Agabian
Sep 1, 1985·Molecular and Biochemical Parasitology·V T ContrerasS Goldenberg
Jan 1, 1984·International Review of Cytology·W de Souza
Oct 1, 1994·Molecular and Biochemical Parasitology·J A BatistaC M de Sá
Jan 30, 2004·Nature Structural & Molecular Biology·Roy Parker, Haiwei Song
May 26, 2004·Biochemical and Biophysical Research Communications·Patrícia A MörkingStenio P Fragoso
Mar 10, 2005·Experimental Parasitology·Bruno DallagiovannaBeatriz Garat
Jun 21, 2005·Current Opinion in Structural Biology·Traci M Tanaka Hall
Jun 21, 2005·Biochemical and Biophysical Research Communications·Florence CaroMartín P Vázquez
Jan 24, 2006·Molecular and Biochemical Parasitology·Jennifer B Palenchar, Vivian Bellofatto
Feb 8, 2006·Experimental Parasitology·Florence CaroMartín P Vázquez
Feb 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Adriana V JägerAlberto C Frasch
Apr 3, 2007·Biochemical and Biophysical Research Communications·Fabíola Barbieri HoletzSamuel Goldenberg
Jul 20, 2007·Molecular Microbiology·Alejandro CassolaAlberto C Frasch
Dec 7, 2007·The Journal of Biological Chemistry·Bruno DallagiovannaMarco A Krieger
Feb 23, 2008·Trends in Biochemical Sciences·Paul Anderson, Nancy Kedersha
Mar 18, 2008·FEBS Letters·Tina GlisovicGideon Dreyfuss
Jun 3, 2008·Current Opinion in Structural Biology·Antoine CléryFrédéric H-T Allain
Jan 23, 2010·Nature Reviews. Molecular Cell Biology·Richard J JacksonTatyana V Pestova
Mar 18, 2010·Parasitology·Peter W CollingridgeMichael L Ginger
Apr 3, 2010·Biochimica Et Biophysica Acta·Daphne H E W HubertsIda J van der Klei
Jan 1, 2009·Progress in Molecular Biology and Translational Science·Nancy Kedersha, Paul Anderson
Jul 16, 2010·The FEBS Journal·Fabíola B HoletzSamuel Goldenberg
Apr 7, 2011·The Journal of Biological Chemistry·Alexandra S LingEdward F Hendriks
Aug 9, 2011·Advances in Parasitology·Samuel Goldenberg, Andrea Rodrigues Avila
Oct 26, 2011·Essays in Biochemistry·Javier G De GaudenziAlejandro Cassola
Mar 20, 2012·Biochemical and Biophysical Research Communications·Eloise P Guerra-SlompoBruno Dallagiovanna
Jul 6, 2012·Cold Spring Harbor Perspectives in Biology·Carolyn J Decker, Roy Parker
Jul 11, 2012·Comparative and Functional Genomics·Rosemary JagusAllen R Place

❮ Previous
Next ❯

Methods Mentioned

BETA
immunoprecipitation
RNA-Seq
two-hybrid
ubiquitination
gene knockout
light
scanning
transfection
flow
PCR

Software Mentioned

LAS AF Leica
FlowJo

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.