RNA biochemistry. Transcriptome-wide distribution and function of RNA hydroxymethylcytosine

Science
Benjamin DelatteFrançois Fuks

Abstract

Hydroxymethylcytosine, well described in DNA, occurs also in RNA. Here, we show that hydroxymethylcytosine preferentially marks polyadenylated RNAs and is deposited by Tet in Drosophila. We map the transcriptome-wide hydroxymethylation landscape, revealing hydroxymethylcytosine in the transcripts of many genes, notably in coding sequences, and identify consensus sites for hydroxymethylation. We found that RNA hydroxymethylation can favor mRNA translation. Tet and hydroxymethylated RNA are found to be most abundant in the Drosophila brain, and Tet-deficient fruitflies suffer impaired brain development, accompanied by decreased RNA hydroxymethylation. This study highlights the distribution, localization, and function of cytosine hydroxymethylation and identifies central roles for this modification in Drosophila.

References

Feb 26, 2004·Nature Genetics·Stephen T ThibaultJonathan Margolis
Jun 16, 2005·Nature Reviews. Molecular Cell Biology·Arianne J MatlinChristopher W J Smith
Dec 17, 2005·Nature·Emmanuelle ViréFrançois Fuks
Jan 21, 2006·Science·Mary Grace GollTimothy H Bestor
May 9, 2006·Trends in Biochemical Sciences·Albert JeltschFrank Lyko
Sep 19, 2008·Genome Biology·Yong ZhangX Shirley Liu
Apr 18, 2009·Science·Skirmantas Kriaucionis, Nathaniel Heintz
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Apr 17, 2010·Science·Assaf ZemachDaniel Zilberman
Apr 15, 2011·Nature·Kristine WilliamsKristian Helin
May 1, 2012·American Journal of Human Genetics·Lia Abbasi-MohebAndreas W Kuss
May 12, 2012·Nature·Dan DominissiniGideon Rechavi
Jan 29, 2013·The EMBO Journal·Rachel DeplusFrançois Fuks
Feb 7, 2013·Nature Reviews. Molecular Cell Biology·Alberto R KornblihttManuel J Muñoz
May 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Günter RaddatzFrank Lyko
Jul 6, 2013·Cell Reports·Andrew R BassettJi-Long Liu
Oct 25, 2013·Nature·Rahul M Kohli, Yi Zhang
Mar 26, 2014·Nature Reviews. Genetics·Ye FuChuan He
Apr 10, 2014·Nature Reviews. Molecular Cell Biology·Kate D Meyer, Samie R Jaffrey
Jul 30, 2014·Journal of the American Chemical Society·Lijuan FuYinsheng Wang
May 6, 2015·Cell·Guoqiang ZhangDahua Chen

❮ Previous
Next ❯

Citations

Jul 21, 2016·Current Opinion in Insect Science·Ryszard Maleszka
Apr 16, 2016·Nucleic Acids Research·Rui WangJia Sheng
Apr 21, 2016·Cellular and Molecular Life Sciences : CMLS·Markus DuechlerBarbara Nawrot
May 31, 2016·DNA Repair·Endalkachew A AlemuArne Klungland
May 29, 2016·Molecular Cancer·Nenad BartonicekMarcel E Dinger
Jun 3, 2016·Wiley Interdisciplinary Reviews. RNA·Patrick A Limbach, Mellie June Paulines
Jun 11, 2016·Acta Neuropathologica·Veronique V BelzilLeonard Petrucelli
Jul 22, 2016·PLoS Genetics·Samantha B SheltonBlerta Xhemalce
Aug 23, 2016·PloS One·Qiang XuQingkai Yang
Sep 7, 2016·Trends in Genetics : TIG·Hélène ScheerDominique Gagliardi
Nov 2, 2016·Epigenetics : Official Journal of the DNA Methylation Society·Sascha H C Duttke
Jun 28, 2016·Wiley Interdisciplinary Reviews. RNA·Thomas Philipp Hoernes, Matthias David Erlacher
Nov 3, 2016·Chembiochem : a European Journal of Chemical Biology·Maria Basanta-SanchezJia Sheng
Dec 30, 2016·Nature Methods·Xiaoyu LiChengqi Yi
Jan 5, 2017·ACS Chemical Biology·Jinghui Song, Chengqi Yi
Dec 19, 2016·Trends in Biotechnology·Danny Incarnato, Salvatore Oliviero
Feb 2, 2017·Nature Reviews. Molecular Cell Biology·Cole J T LewisAuinash Kalsotra
Jan 21, 2017·Journal of the American Chemical Society·Sabrina M HuberShankar Balasubramanian
Jan 21, 2017·Journal of the American Society for Mass Spectrometry·Mellie June Paulines, Patrick A Limbach
Apr 26, 2017·RNA Biology·Franziska R Traube, Thomas Carell
Mar 30, 2017·Chemical Society Reviews·Yuqi ChenXiang Zhou
Jan 20, 2017·Nature·Emily M HarcourtEric T Kool
Oct 27, 2017·Nucleic Acids Research·Satoshi KimuraTsutomu Suzuki
Dec 16, 2017·Genes, Brain, and Behavior·Y Jung, D Goldman
Sep 20, 2017·Genes, Brain, and Behavior·L J LeightonT W Bredy
Sep 25, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sebastian CanovasPilar Coy
Jun 20, 2018·Wiley Interdisciplinary Reviews. RNA·Helena Covelo-MolaresStepanka Vanacova
Nov 3, 2016·Development·Michaela Frye, Sandra Blanco
Jul 13, 2016·Molecular and Cellular Biology·Sergi ArandaLuciano Di Croce
Oct 13, 2018·Wiley Interdisciplinary Reviews. RNA·Lukas Trixl, Alexandra Lusser
Oct 30, 2018·Angewandte Chemie·Virginie MarchandYuri Motorin
Sep 20, 2018·Annual Review of Genetics·Sigrid Nachtergaele, Chuan He
Oct 23, 2018·Chemistry : a European Journal·Katja Hartstock, Andrea Rentmeister
Feb 15, 2018·Nature Chemical Biology·Anya V Grozhik, Samie R Jaffrey
Nov 23, 2017·Nature Reviews. Molecular Cell Biology·Kathrin LeppekMaria Barna
May 28, 2019·Stem Cell Reviews and Reports·Varun Haran, Nibedita Lenka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.