PMID: 11927255Apr 3, 2002Paper

RNA-catalyzed RNA ligation on an external RNA template

Chemistry & Biology
Kathleen E McGinness, G F Joyce

Abstract

Variants of the hc ligase ribozyme, which catalyzes ligation of the 3' end of an RNA substrate to the 5' end of the ribozyme, were utilized to evolve a ribozyme that catalyzes ligation reactions on an external RNA template. The evolved ribozyme catalyzes the joining of an oligonucleotide 3'-hydroxyl to the 5'-triphosphate of an RNA hairpin molecule. The ribozyme can also utilize various substrate sequences, demonstrating a largely sequence-independent mechanism for substrate recognition. The ribozyme also carries out the ligation of two oligonucleotides that are bound at adjacent positions on a complementary template. Finally, it catalyzes addition of mononucleoside 5'-triphosphates onto the 3' end of an oligonucleotide primer in a template-dependent manner. The development of ribozymes that catalyze polymerase-type reactions contributes to the notion that an RNA world could have existed during the early history of life on Earth.

References

Aug 1, 1992·PCR Methods and Applications·R C Cadwell, G F Joyce
Aug 30, 1990·Nature·A D Ellington, J W Szostak
Apr 5, 1986·Journal of Molecular Biology·G F Joyce, L E Orgel
Jan 1, 1987·Cold Spring Harbor Symposia on Quantitative Biology·G F Joyce
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A M Weiner, N Maizels
Sep 25, 1984·Journal of Molecular Biology·T InoueC B Reese
Dec 2, 1982·Nature·J Abelson
Jun 25, 1984·Journal of Molecular Biology·G F JoyceL E Orgel
Dec 25, 1980·Journal of Molecular Biology·P K Bridson, L E Orgel
Jan 1, 1994·Nature Structural Biology·S A Strobel, T R Cech
Jun 24, 1994·Science·J R PrudentP G Schultz
Oct 11, 1993·Nucleic Acids Research·F G Pluthero
Jul 25, 1996·Nature·E H Ekland, D P Bartel
Oct 23, 1997·Chemistry & Biology·T W WiegandB E Eaton
Apr 16, 1998·Science·D K TreiberJ R Williamson
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·L F Landweber, I D Pokrovskaya
Apr 17, 1999·American Journal of Human Genetics·N Maizels
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·L JaegerG F Joyce
Mar 29, 2000·Nucleic Acids Research·M P Robertson, A D Ellington

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Citations

Aug 16, 2006·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Wentao Ma, Chunwu Yu
Jul 12, 2002·Nature·Gerald F Joyce
Jun 11, 2004·Annual Review of Biochemistry·Gerald F Joyce
Jul 1, 2005·RNA·Michael S Lawrence, David P Bartel
Mar 13, 2014·Annual Review of Biochemistry·J Craig Blain, Jack W Szostak
Sep 15, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yoshiya IkawaTan Inoue
Sep 9, 2008·The International Journal of Biochemistry & Cell Biology·Andrew D EllingtonAngel Syrett
Oct 4, 2006·Bio Systems·Wentao MaWentao Zhang
Feb 27, 2013·Chemistry & Biology·Nilesh VaidyaNiles Lehman
Aug 2, 2003·Chemistry & Biology·Hiroshi MurakamiHiroaki Suga
Aug 26, 2006·Chemistry & Biology·Eric J Hayden, Niles Lehman
Apr 28, 2006·Chemistry & Biology·Natasha PaulGerald F Joyce
Feb 8, 2003·Chemistry & Biology·Kathleen E McGinness, Gerald F Joyce
Nov 1, 2013·Chemical Reviews·Kepa Ruiz-MirazoAndrés de la Escosura
Sep 28, 2017·ELife·Biswajit Samanta, Gerald F Joyce
Nov 5, 2014·Nature·Jonathan T Sczepanski, Gerald F Joyce
Jan 29, 2003·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Sandeep VermaMichael Famulok
Jan 1, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuki FujitaYoshiya Ikawa
Jul 20, 2007·Angewandte Chemie·Gerald F Joyce
Mar 4, 2020·Proceedings of the National Academy of Sciences of the United States of America·Travis WaltonJack W Szostak
Sep 6, 2018·Cold Spring Harbor Perspectives in Biology·Gerald F Joyce, Jack W Szostak

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