PMID: 7513426Apr 26, 1994Paper

RNA cleavage and chain elongation by Escherichia coli DNA-dependent RNA polymerase in a binary enzyme.RNA complex

Proceedings of the National Academy of Sciences of the United States of America
C R AltmannM J Chamberlin

Abstract

In the absence of DNA, Escherichia coli RNA polymerase (EC 2.7.7.6) can bind RNA to form an equimolar binary complex with the concomitant release of the sigma factor. We show now that E. coli RNA polymerase binds at a region near the 3' terminus of the RNA and that an RNA in such RNA.RNA polymerase complexes undergoes reactions previously thought to be unique to nascent RNA in ternary complexes with DNA. These include GreA/GreB-dependent cleavage of the RNA and elongation by 3'-terminal addition of NMP from NTP. Both of these reactions are inhibited by rifampicin. Hence, by several criteria, the RNA in binary complexes is bound to the polymerase in a manner quite similar to that in ternary complexes. These findings can be explained by a model for the RNA polymerase ternary complex in which the RNA is bound at the 3' terminus through two protein binding sites located up to 10 nt apart. In this model, the stability of RNA binding to the polymerase in the ternary complex is due primarily to its interaction with the protein.

References

Aug 1, 1977·Archives of Biochemistry and Biophysics·N GonzalezM J Chamberlin
Aug 15, 1979·European Journal of Biochemistry·A SpasskyH Buc
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S BorukhovA Goldfarb
Sep 1, 1992·Trends in Biochemical Sciences·N Usman, R Cedergren
Aug 31, 1990·Biophysical Chemistry·S C GillP H von Hippel
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P H von Hippel, T D Yager
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·C K SurrattM J Chamberlin
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J A JaegerM Zuker
Nov 11, 1987·Nucleic Acids Research·J F MilliganO C Uhlenbeck
Dec 1, 1967·European Journal of Biochemistry· FuchseG Walter
Mar 1, 1966·Journal of Molecular Biology·H BremerM Konrad
Mar 1, 1983·European Journal of Biochemistry·M Huaifeng, G R Hartmann
Jan 25, 1984·Journal of Molecular Biology·M G Fried, D M Crothers
Feb 12, 1993·Science·G A Kassavetis, E P Geiduschek
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D Wang, D K Hawley
Feb 12, 1993·Cell·S BorukhovA Goldfarb
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D Reines, J Mote
Jul 1, 1963·Journal of Molecular Biology·A TISSIERESF GROS

❮ Previous
Next ❯

Citations

Aug 2, 2007·Journal of Applied Genetics·Agnieszka Szalewska-PalaszAlicja Wegrzyn
Feb 1, 1995·Current Opinion in Structural Biology·E ArnoldZ Hostomsky
Feb 11, 2000·RNA·J Filipovska, M M Konarska
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·M D RuddD S Luse
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·A MustaevA Goldfarb
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·W GuD Reines
Dec 21, 2004·European Journal of Biochemistry·Andrey KulbachinskiyVadim Nikiforov
Jan 11, 2002·Molecular and Cellular Biology·Jennifer F Kugel, James A Goodrich
Jan 1, 1997·Annual Review of Biochemistry·S M UptainM J Chamberlin
Jun 6, 2009·Annual Review of Biochemistry·Evgeny Nudler
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·S M Uptain, M J Chamberlin
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·S S Daube, P H von Hippel
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·N Komissarova, M Kashlev
Apr 5, 2013·PLoS Pathogens·Sylvie ChenavasRob W H Ruigrok
May 18, 1999·Journal of Molecular Biology·E Nudler
Mar 28, 1997·The Journal of Biological Chemistry·S S Sastry, B M Ross
Mar 14, 1997·The Journal of Biological Chemistry·D KoulichS Borukhov
Feb 21, 2002·The Journal of Biological Chemistry·Motoki SusaNobuo Shimamoto
May 21, 1999·The Journal of Biological Chemistry·F J López de SaroJ D Helmann
May 10, 2000·The Journal of Biological Chemistry·M V Sukhodolets, D J Jin
Apr 18, 1998·The Journal of Biological Chemistry·M V Sukhodolets, D J Jin
Apr 15, 2003·The Journal of Biological Chemistry·Rodney G WeilbaecherCaroline M Kane
Aug 11, 1995·The Journal of Biological Chemistry·D R ChafinD H Price
Feb 22, 2002·The Journal of Biological Chemistry·Mikhail Kashlev, Natalia Komissarova
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Aug 14, 1998·Molecular Cell·I SidorenkovM Kashlev
Dec 13, 2006·Science·Karen M Wassarman, Ruth M Saecker
Aug 5, 1994·Science·E NudlerM Kashlev

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.