RNA Control by Photoreversible Acylation

Journal of the American Chemical Society
Willem A VelemaEric T Kool

Abstract

External photocontrol over RNA function has emerged as a useful tool for studying nucleic acid biology. Most current methods rely on fully synthetic nucleic acids with photocaged nucleobases, limiting application to relatively short synthetic RNAs. Here we report a method to gain photocontrol over RNA by postsynthetic acylation of 2'-hydroxyls with photoprotecting groups. One-step introduction of these groups efficiently blocks hybridization, which is restored after light exposure. Polyacylation (termed cloaking) enables control over a hammerhead ribozyme, illustrating optical control of RNA catalytic function. Use of the new approach on a transcribed 237 nt RNA aptamer demonstrates the utility of this method to switch on RNA folding in a cellular context, and underlines the potential for application in biological studies.

Citations

Apr 13, 2019·Chemical Communications : Chem Comm·Hyun Shin ParkEric T Kool
Jan 5, 2020·Nature Communications·Shao-Ru WangXiang Zhou
Sep 29, 2020·Nature Reviews. Chemistry·Willem A Velema, Eric T Kool
Jul 24, 2020·International Journal of Molecular Sciences·Olga A KrashenininaAlya G Venyaminova
Jul 17, 2020·Advanced Science·Shao-Ru WangXiang Zhou
Nov 10, 2020·Advanced Materials·Alexandra E RangelH Tom Soh
Feb 13, 2021·Bioscience, Biotechnology, and Biochemistry·Takeharu MinoItaru Hamachi
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Linlin Yang, Ivan J Dmochowski
Oct 6, 2020·Journal of the American Chemical Society·Steve D KnutsonJennifer M Heemstra
Apr 20, 2021·Chemistry : a European Journal·Song MaoJia Sheng
Jul 12, 2020·Current Opinion in Chemical Biology·Yuting WuYi Lu
Jul 29, 2021·Chemical Society Reviews·Alicia E Mangubat-Medina, Zachary T Ball
Oct 10, 2018·Organic Letters·Willem A Velema, Eric T Kool
May 8, 2020·ACS Chemical Biology·Shaoru WangXiang Zhou
Sep 1, 2020·Journal of the American Chemical Society·Lu XiaoEric T Kool
Jul 4, 2019·Organic Letters·Maryam HabibianEric T Kool
Oct 9, 2021·Angewandte Chemie·Lu XiaoEric T Kool

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