PMID: 46286Mar 1, 1975

RNA-dependent DNA polymerase activity of RNA tumor viruses. V. Rous sarcoma virus single-stranded RNA-DNA covalent hybrids in infected chicken embryo fibroblast cells

Journal of Virology
J LeisJ Hurwitz


RNA-DNA covalent hybrids containing viral RNA have been isolated from nuclear fractions of Rous sarcoma virus-infected chicken embryo fibroblast cells shortly after virus infection. The formation of covalent hybrid structures depends upon a functional reverse transcriptase in vivo, since its appearance in cells is temperature dependent when infected with Rous sarcoma virus mutant LA335, which contains a temperature-sensitive reverse transcriptase.


Dec 1, 1975·CRC Critical Reviews in Biochemistry·A M Wu, R C Gallo
Dec 15, 1975·FEBS Letters·A M Holmes, I R Johnston

Related Concepts

Chicken Tumor 1 Virus
Centrifugation, Isopycnic
Centrifugation, Zonal
Chick Embryo
DNA, Double-Stranded
Genomic Hybridization
RNA-Directed DNA Polymerase
RNA, Viral

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