RNA-dependent RNA polymerase (NIb) of the potyviruses is an avirulence factor for the broad-spectrum resistance gene Pvr4 in Capsicum annuum cv. CM334

PloS One
Saet-Byul KimDoil Choi

Abstract

Potyviruses are one of the most destructive viral pathogens of Solanaceae plants. In Capsicum annuum landrace CM334, a broad-spectrum gene, Pvr4 is known to be involved in resistance against multiple potyviruses, including Pepper mottle virus (PepMoV), Pepper severe mosaic virus (PepSMV), and Potato virus Y (PVY). However, a potyvirus avirulence factor against Pvr4 has not been identified. To identify the avirulence factor corresponding to Pvr4 in potyviruses, we performed Agrobacterium-mediated transient expressions of potyvirus protein coding regions in potyvirus-resistant (Pvr4) and -susceptible (pvr4) pepper plants. Hypersensitive response (HR) was observed only when a RNA-dependent RNA polymerase (NIb) of PepMoV, PepSMV, or PVY was expressed in Pvr4-bearing pepper leaves in a genotype-specific manner. In contrast, HR was not observed when the NIb of Tobacco etch virus (TEV), a virulent potyvirus, was expressed in Pvr4-bearing pepper leaves. Our results clearly demonstrate that NIbs of PepMoV, PepSMV, and PVY serve as avirulence factors for Pvr4 in pepper plants.

References

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Citations

Nov 16, 2017·Molecular Plant-microbe Interactions : MPMI·Joo Hyun LeeDoil Choi
Aug 13, 2020·Molecular Plant-microbe Interactions : MPMI·Solhee InDoil Choi
Sep 10, 2016·The New Phytologist·Saet-Byul KimByoung-Cheorl Kang
Jul 10, 2019·Molecular Plant Pathology·Hernan Garcia-Ruiz
Sep 12, 2018·Viruses·Hernan Garcia-Ruiz
Jan 21, 2017·Frontiers in Microbiology·Bianca C GouveiaElizabeth P B Fontes
Apr 24, 2021·Frontiers in Plant Science·Brian T RossMichelle L Flenniken
Apr 24, 2021·Annual Review of Phytopathology·Xiuling YangAiming Wang

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Methods Mentioned

BETA
PCR
ELISA
Assay

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