RNA fingerprinting--a new method to screen for differences in plant litter degrading microbial communities

Journal of Microbiological Methods
Manish Kumar AnejaMichael Schloter

Abstract

Microbial activities are essential for the nutrient turnover processes in soil and play an important role in the degradation of complex organic material, for example, plant leaf litter. However, very little is known about the microorganisms and their genes involved during the course of leaf litter decomposition. In the present study, we describe the non-radioactive application of RNA arbitrarily primed-PCR (RAP-PCR) protocol in combination with the classic litter bag technique to investigate the metabolic profiles of microbial community involved in leaf litter degradation after 2 and 8 weeks of degradation in four different soil sites, without using selective primer systems for PCR. Due to the significantly reduced target sites for PCR primers, compared to the published papers about RAP fingerprinting of more complex microbial communities based on DNA analysis (only transcripts from microbes on the litter material were analysed), the patterns of parallel samples were highly reproducible (>95%). Shifts in microbial community structure and function were observed during the course of degradation. Each litter sample had its unique metabolic profile and both soil effects and litter quality effects were evident. RAP-PCR products were...Continue Reading

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Citations

Mar 9, 2007·Microbial Ecology·Andreas NockerAnne K Camper
Apr 7, 2005·Applied and Environmental Microbiology·Shilpi SharmaMichael Schloter
Mar 7, 2006·Applied and Environmental Microbiology·Shilpi SharmaMichael Schloter
Dec 5, 2008·FEMS Microbiology Reviews·José M VieitesManuel Ferrer
Nov 30, 2004·FEMS Microbiology Letters·S SharmaJ C Munch
Nov 22, 2008·Environmental Monitoring and Assessment·Surindra SutharSushma Singh

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