PMID: 7538661Apr 25, 1995Paper

RNA helicase activity of the plum pox potyvirus CI protein expressed in Escherichia coli. Mapping of an RNA binding domain

Nucleic Acids Research
A FernándezJ A García

Abstract

The plum pox potyvirus (PPV) cylindrical inclusion (CI) protein fused to the maltose binding protein (MBP) has been synthesized in Escherichia coli and purified by affinity chromatography in amylose resin. In the absence of any other viral factors, the fusion product had NTPase, RNA binding and RNA helicase activities. These in vitro activities were not affected by removal of the last 103 amino acids of the CI protein. However, other deletions in the C-terminal part of the protein, although leaving intact all the region conserved in RNA helicases, drastically impaired the ability to unwind dsRNA and to hydrolyze NTPs. A mutant protein lacking the last 225 residues retained the competence to interact with RNA. Further deletions mapped boundaries of the RNA binding domain within residues 350 and 402 of the PPV CI protein. This region includes the arginine-rich motif VI, the most carboxy terminal conserved domain of RNA helicases of the superfamily SF2. These results indicate that NTP hydrolysis is not an essential component for RNA binding of the PPV CI protein.

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Citations

Feb 28, 2001·Virus Research·S Urcuqui-InchimaF Bernardi
Jul 22, 1998·The Plant Journal : for Cell and Molecular Biology·J C CarringtonM C Schaad
Aug 17, 2001·Journal of Virology·K Hirashima, Y Watanabe
Jan 11, 2014·Molecular Plant-microbe Interactions : MPMI·M SorelS German-Retana
Sep 25, 1998·Critical Reviews in Biochemistry and Molecular Biology·A LükingU Schmidt
Apr 27, 1999·FEBS Letters·M Gomez de CedrónL Kääriäinen
Oct 25, 2011·Molecular Plant Pathology·Karen-Beth G ScholthofGary D Foster
Oct 10, 2013·Molecular Plant Pathology·Juan Antonio GarcíaThierry Candresse
Nov 1, 2005·Virus Research·Marta Gómez de CedrónJuan Antonio García
Apr 24, 2014·TheScientificWorldJournal·Md Abul Kalam AzadNik Marzuki Sidik
Nov 22, 2014·Frontiers in Plant Science·Asako UchiyamaSondra G Lazarowitz
Dec 14, 1999·Molecular Microbiology·M C Hall, S W Matson
Apr 25, 1997·The Journal of Biological Chemistry·S Zhang, F Grosse
Apr 1, 1997·Molecular and Cellular Biology·Z J LorkovićR Oelmüller
Apr 1, 1997·Journal of Virology·G Kadaré, A L Haenni

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