RNA in the nucleus of a motile plant spermatozoid: characterization by enzyme-gold cytochemistry and in situ hybridization.

Molecular Reproduction and Development
E RejonD Robert

Abstract

To investigate the decrease of transcription and to determine the localization of RNA molecules during spermiogenesis in the fern Scolopendrium, we have used the enzyme-gold electron microscope method. During cell differentiation, the labeling decreases over the cytoplasm and increases over the nucleus. In the latter, the nucleolus disappears at the beginning of differentiation and the gold particles that were at first situated over the dispersed chromatin are then located over the condensed chromatin. In mature gametes, gold particles linked to RNA are abundant over the dense and homogeneous nucleus and sparse over the cytoplasm. By using in situ hybridization of RNA-poly(A) tails with radioactive poly(U) probes, we have shown that these RNA molecules are mRNA; they are located in the condensed chromatin of the mature sperm nucleus.

References

Oct 15, 1978·Experimental Cell Research·W F Reynolds, S L Wolfe
Jan 1, 1982·Developmental Biology·D G Capco, W R Jeffrey

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Citations

Sep 1, 1991·Molecular Reproduction and Development·J KuligowskiE Chenou
Apr 13, 2005·Trends in Molecular Medicine·David MillerStephen A Krawetz
Sep 2, 2005·Nature Reviews. Genetics·Stephen A Krawetz
Jul 17, 2013·Human Reproduction Update·Meritxell JodarUNKNOWN Reproductive Medicine Network
May 18, 2005·Asian Journal of Andrology·Rui Pires Martins, Stephen A Krawetz
Feb 24, 2006·Asian Journal of Andrology·Benjamin R Emery, Douglas T Carrell
Aug 27, 2015·Asian Journal of Andrology·Zi-Jue ZhuZheng Li
Mar 14, 2009·Microscopy Research and Technique·Jean-Pierre Dadoune
Dec 6, 2007·Reproduction in Domestic Animals = Zuchthygiene·D Miller
Mar 8, 2018·Biology of Reproduction·Marta GòdiaStephen A Krawetz
Aug 3, 2006·Human Reproduction Update·David Miller, G Charles Ostermeier

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