RNA interference as a key to knockdown overexpressed cyclooxygenase-2 gene in tumour cells.

British Journal of Cancer
Antonio StrillacciVittorio Tomasi

Abstract

Silencing those genes that are overexpressed in cancer and contribute to the survival and progression of tumour cells is the aim of several researches. Cyclooxygenase-2 (COX-2) is one of the most intensively studied genes since it is overexpressed in most tumours, mainly in colon cancer. The use of specific COX-2 inhibitors to treat colon cancer has generated great enthusiasm. Yet, the side effects of some inhibitors emerging during long-term treatment have caused much concern. Genes silencing by RNA interference (RNAi) has led to new directions in the field of experimental oncology. In this study, we detected sequences directed against COX-2 mRNA, that potently downregulate COX-2 gene expression and inhibit phorbol 12-myristate 13-acetate-induced angiogenesis in vitro in a specific, nontoxic manner. Moreover, we found that the insertion of a specific cassette carrying anti-COX-2 short hairpin RNA sequence into a viral vector (pSUPER.retro) greatly increased silencing potency in a colon cancer cell line (HT29) without activating any interferon response. Phenotypically, COX-2 deficient HT29 cells showed a significant impairment of their in vitro malignant behaviour. Thus, the retroviral approach enhancing COX-2 knockdown, mediat...Continue Reading

References

Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Jan 5, 2000·Genes & Development·T TuschlP A Sharp
Feb 7, 2001·Genes & Development·S M ElbashirT Tuschl
Jan 12, 2002·Diseases of the Colon and Rectum·Takeyoshi YamauchiMasaki Kitajima
Mar 20, 2002·Nature Reviews. Cancer·R A Gupta, R N Dubois
Mar 23, 2002·Science·Thijn R BrummelkampReuven Agami
Jun 11, 2002·Methods : a Companion to Methods in Enzymology·Sayda M ElbashirThomas Tuschl
Sep 21, 2002·Cancer Cell·Thijn R BrummelkampReuven Agami
Nov 14, 2002·Annual Review of Genetics·Marcel TijstermanRonald H A Plasterk
Mar 4, 2003·Nature Reviews. Drug Discovery·Rod J Flower
Mar 5, 2003·Trends in Molecular Medicine·Miguel A IñiguezJuan Miguel Redondo
Jun 11, 2003·Nature Genetics·Alan J BridgeRichard Iggo
Aug 28, 2003·Nature Cell Biology·Carol A SledzBryan R G Williams
Sep 23, 2003·Journal of Cellular Physiology·Enzo SpisniVittorio Tomasi
Nov 5, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mario Romano, Joan Claria
Dec 4, 2003·International Journal of Cancer. Journal International Du Cancer·Stefania BeniniKatia Scotlandi
Feb 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·George Adrian CalinCarlo M Croce
Mar 3, 2004·Nature Biotechnology·Dong-Ho KimJohn J Rossi
Sep 17, 2004·Nature·Craig C Mello, Darryl Conte
Sep 17, 2004·Nature·Gunter Meister, Thomas Tuschl
Oct 8, 2004·The New England Journal of Medicine·Garret A Fitzgerald
Nov 19, 2004·The New England Journal of Medicine·Felix Oberholzer-Gee, S Noorein Inamdar
Nov 24, 2004·Nature Biotechnology·Jeremy D HeidelMark E Davis
Mar 22, 2005·Nature Biotechnology·Adam D JudgeIan MacLachlan
Jun 10, 2005·Nature·Lin HeScott M Hammond
Jun 10, 2005·Nature·Jun LuTodd R Golub
Dec 7, 2005·Journal of Cancer Research and Clinical Oncology·Joong-Won ParkChang-Min Kim
Dec 27, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Melissa M Brierley, Eleanor N Fish

❮ Previous
Next ❯

Citations

Mar 27, 2010·International Journal of Cell Biology·Cyril SobolewskiMarc Diederich
Jul 14, 2010·Journal of Biomedicine & Biotechnology·Antonio StrillacciEnzo Spisni
Nov 14, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Enzo SpisniAntonio Strillacci
Nov 20, 2008·Journal of Cellular and Molecular Medicine·Pasquale SansoneMassimiliano Bonafè
Apr 5, 2013·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Jose PradosAntonia Aranega
Oct 12, 2019·World Journal of Gastroenterology : WJG·Li WeiJiang-Long Huang
Oct 13, 2021·ACS Nano·Esther H RohMillicent O Sullivan

❮ Previous
Next ❯

Methods Mentioned

BETA
antisense oligonucleotides
transfection
FCS
ELISA
confocal microscopy
electrophoresis
PCR
flow cytometry
PMA
light microscopy

Software Mentioned

PROLIGO
BLAST
Image Master VDS
Leica TCS
Beacon Designer

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Biomedicine & Biotechnology
Antonio StrillacciEnzo Spisni
Biomolecular Engineering
Andrei L Gartel, Eugene S Kandel
International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society
J S LeeK W Min
© 2022 Meta ULC. All rights reserved