RNA interference of NADPH-cytochrome P450 reductase results in reduced insecticide resistance in the bed bug, Cimex lectularius.

PloS One
Fang ZhuSubba R Palli

Abstract

NADPH-cytochrome P450 reductase (CPR) plays a central role in cytochrome P450 action. The genes coding for P450s are not yet fully identified in the bed bug, Cimex lectularius. Hence, we decided to clone cDNA and knockdown the expression of the gene coding for CPR which is suggested to be required for the function of all P450s to determine whether or not P450s are involved in resistance of bed bugs to insecticides. The full length Cimex lectularius CPR (ClCPR) cDNA was isolated from a deltamethrin resistant bed bug population (CIN-1) using a combined PCR strategy. Bioinformatics and in silico modeling were employed to identify three conserved binding domains (FMN, FAD, NADP), a FAD binding motif, and the catalytic residues. The critical amino acids involved in FMN, FAD, NADP binding and their putative functions were also analyzed. No signal peptide but a membrane anchor domain with 21 amino acids which facilitates the localization of ClCPR on the endoplasmic reticulum was identified in ClCPR protein. Phylogenetic analysis showed that ClCPR is closer to the CPR from the body louse, Pediculus humanus corporis than to the CPRs from the other insect species studied. The ClCPR gene was ubiquitously expressed in all tissues tested bu...Continue Reading

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Citations

Feb 3, 2016·Nature Communications·Joshua B BenoitStephen Richards
Dec 22, 2015·Environmental Science and Pollution Research International·Lisa LalouetteDavid Siaussat
Jun 4, 2015·Scientific Reports·Jennifer R GordonKenneth F Haynes
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Nov 3, 2015·Scientific Reports·Feng Liu, Nannan Liu
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Apr 21, 2016·PloS One·Minoru MoriyamaTakema Fukatsu
Jan 15, 2021·Scientific Reports·Christos G AthanassiouChristos T Nakas

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Datasets Mentioned

BETA
JQ178363

Methods Mentioned

BETA
PCR

Software Mentioned

TASSER
Compute
SAS
M E volutionary G enetic A nalysis
ClustalW
Bestkeeper
MEGA
Protein Hydrophobicity Plots
BLAST
PHYRE2

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