RNA interference of timeless gene does not disrupt circadian locomotor rhythms in the cricket Gryllus bimaculatus

Journal of Insect Physiology
Yoshiki DanbaraKenji Tomioka

Abstract

Molecular studies revealed that autoregulatory negative feedback loops consisting of so-called "clock genes" constitute the circadian clock in Drosophila. However, this hypothesis is not fully supported in other insects and is thus to be examined. In the cricket Gryllus bimaculatus, we have previously shown that period (per) plays an essential role in the rhythm generation. In the present study, we cloned cDNA of the clock gene timeless (tim) and investigated its role in the cricket circadian oscillatory mechanism using RNA interference. Molecular structure of the cricket tim has rather high similarity to those of other insect species. Real-time RT-PCR analysis revealed that tim mRNA showed rhythmic expression in both LD and DD similar to that of per, peaking during the (subjective) night. When injected with tim double-stranded RNA (dstim), tim mRNA levels were significantly reduced and its circadian expression rhythm was eliminated. After the dstim treatment, however, adult crickets showed a clear locomotor rhythm in DD, with a free-running period significantly shorter than that of control crickets injected with Discosoma sp. Red2 (DsRed2) dsRNA. These results suggest that in the cricket, tim plays some role in fine-tuning of ...Continue Reading

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Citations

Feb 14, 2012·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Kenji TomiokaTaishi Yoshii
Feb 26, 2013·Behavior Genetics·Daniel J Fergus, Kerry L Shaw
Jan 9, 2014·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Roman V FrolovMatti Weckström
Oct 7, 2014·Zoological Science·Kenji Tomioka
Mar 6, 2013·Journal of Insect Physiology·Outa UryuTaishi Yoshii
Dec 12, 2012·Journal of Biological Rhythms·Achim WerckenthinMonika Stengl
Dec 12, 2012·Journal of Biological Rhythms·Yoshiyuki MoriyamaKenji Tomioka
Jan 30, 2014·Memórias do Instituto Oswaldo Cruz·Carla GentileAlexandre Afranio Peixoto
Nov 3, 2017·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Octavio Manuel Palacios-GimenezDiogo Cavalcanti Cabral-de-Mello
Dec 18, 2020·Frontiers in Behavioral Neuroscience·Katharina Beer, Charlotte Helfrich-Förster
Dec 10, 2020·Zoological Letters·Yasuaki TomiyamaKenji Tomioka

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