RNA interference screening demystified.

Journal of Clinical Pathology
Christopher J LordAlan Ashworth

Abstract

Genetic screens, where the effects of modifying gene function on cell behaviour are assessed in a systematic fashion, have for some time provided useful information to those interested in disease pathogenesis and treatment. Genetic screens exploiting the phenomenon of RNA interference (RNAi) are now becoming commonplace. This article explains the different RNAi screen formats and describes some of the applications of RNAi screening that may be pertinent to the research pathologist.

References

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Citations

May 30, 2013·Journal of Proteome Research·Veronika A GlukhovaMichael J MacCoss
Dec 3, 2009·Briefings in Functional Genomics·Jaakko Mattila, Oscar Puig
Apr 30, 2011·Briefings in Functional Genomics·Ming JiangMichael Howell
Jul 28, 2011·Briefings in Functional Genomics·Eric Campeau, Stéphane Gobeil
Apr 7, 2010·Annual Review of Biochemistry·Stephanie MohrNorbert Perrimon
Feb 18, 2016·PloS One·Sara Costa-CabralAlan Ashworth
Jul 2, 2016·Molecular Cancer Therapeutics·Rowan E MillerAlan Ashworth

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Methods Mentioned

BETA
transfection
transfect
PCR
dissection

Software Mentioned

CellHTS
Ingenuity

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