RNA interference‑mediated knockdown of translationally controlled tumor protein induces apoptosis, and inhibits growth and invasion in glioma cells

Molecular Medicine Reports
Hua JinLizhuang Yang

Abstract

Translationally controlled tumor protein (TCTP) is a highly conserved, growth‑associated and small molecule protein, which is highly expressed in various types of tumor cell. TCTP can promote the growth and suppress apoptosis of tumor cels. However, few studies have reported the effects of TCTP in gliomas. In the present study, a glioma cell line was established, which was stably transfected with TCTP short hairpin ribonucleic acid (shRNA), to investigate the impact of downregulated expression of TCTP on the proliferation, apoptosis and invasion of glioma cells. Western blot and reverse transcription-quantitative polymerase chain reaction analyses demonstrated that TCTP shRNA effectively reduced the expression of TCTP in the U251 glioma cell line. MTT and colony formation assays revealed that downregulated expression of TCTP significantly inhibited glioma cell proliferation. Cell cycle analysis using flow cytometry revealed that the cells in the pRNA‑H1.1‑TCTP group were arrested in the G0/G1 phase of the cell cycle. Western blot analysis detected downregulated expression levels of cyclins, including Cyclin D1, Cyclin E and Cyclin B. Annexin V‑fluorescein isothiocyanate/propidium iodide and Hoechst staining demonstrated that th...Continue Reading

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Citations

Sep 1, 2020·Biotechnology and Bioengineering·Camila A OrellanaEsteban Marcellin
Oct 1, 2020·Clinical and Translational Medicine·Shanqiang QuHuafu Wang
Mar 13, 2016·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Shuai HaoChengtao Wang

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Methods Mentioned

BETA
Protein Assay
flow cytometry
zymography
antisense oligonucleotide

Software Mentioned

Pro
GraphPad
Graphpad Prism
Gel
- Analyzer

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