Jun 16, 1975

RNA metabolism in nuclei isolated from HeLa cells

European Journal of Biochemistry
E Busiello, M Di Girolamo

Abstract

Nuclei with low cytoplasmic contamination, capable of synthesizing RNA for an extended period of time, were prepared from HeLa cells. Besides elongating RNA chains already initiated in vivo, the nuclear preparation initiates the synthesis of new RNA chains. This was shown by labelling the newly synthesized RNA with [gamma-32P]GTP and by detecting the presence of labelled guanosine tetraphosphate among the alkaline hydrolysis products of synthesized RNA. By synthesizing RNA in the presence of each of the four gamma-32P-labelled nucleoside triphosphates, it was possible to conclude that RNA chain synthesis starts predominantly with a purine base. Both nucleolar and nucleoplasmic RNAs are made. The nuclear preparation methylates the nucleolar RNA by utilizing S-adenosyl-L-methionine as a methyl-group donor.

Mentioned in this Paper

Amanitins
Manganese
Transcription, Genetic
Guanosine Tetraphosphate
Potassium
Cell Nucleus
RNA Metabolism
Cytoplasmic
Nucleolar
Sodium

About this Paper

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