PMID: 7540096May 1, 1995Paper

RNA metabolism in situ at the 93D heat shock locus in polytene nuclei of Drosophila melanogaster after various treatments

Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology
S C Lakhotia, A Sharma

Abstract

Quantitative in situ hybridization to RNA on polytene chromosome spreads, using the 93D exon-, intron- and repeat-specific 35S-labeled antisense RNA probes, revealed treatment- (heat shock, benzamide, colchicine, heat shock followed by benzamide and heat shock in the presence of colchicine) specific differences in the metabolism (synthesis and/or accumulation at the puff site) of the various hsr-omega transcripts, namely hsr-omega-nuclear (omega-n), omega-pre-cytoplasmic (omega-pre-c) and omega-cytoplasmic (omega-c). While heat shock increased the levels of all the three transcripts at the 93D puff site in a coordinated manner, benzamide led to a significant increase in the levels of hsr-omega-n and pre-c; on the other hand, colchicine caused increased levels of the omega-n and omega-c RNA species at 93D. The results also suggested splicing of hsr-omega-pre-c RNA at the site of synthesis with the spliced-out 'free' intron (hsr-omega-fi) accumulating at the puff site. The rate of splicing and/or turnover of the hsr-omega-fi varied in a treatment-specific manner. Although a combined treatment to salivary glands with heat shock and benzamide or colchicine is known to inhibit puffing and [3H]uridine incorporation at 93D, the two tr...Continue Reading

References

Feb 1, 1992·Current Opinion in Cell Biology·R H Singer
Nov 1, 1990·Molecular and Cellular Biology·M F LeMaire, C S Thummel
Jan 1, 1989·Genome Génome / Conseil National De Recherches Canada·S C Lakhotia
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Sep 1, 1995·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·A Sharma, S C Lakhotia
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Citations

Sep 1, 1995·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·A Sharma, S C Lakhotia
Jul 30, 2011·Journal of Biosciences·Subhash C Lakhotia
Mar 13, 2009·Cell Stress & Chaperones·Travis K JohnsonStephen W McKechnie
Dec 10, 1998·Nucleic Acids Research·V A ErdmannJ Barciszewski
Mar 28, 2017·Experimental Neurology·Luca Lo Piccolo, Masamitsu Yamaguchi
Feb 18, 2017·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Luca Lo PiccoloMaria Cristina Onorati
Aug 15, 2008·Biotechnology Journal·Elena Savvateeva-PopovaMichael Evgen'ev

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