RNA polymerase activity catalyzed by a potyvirus-encoded RNA-dependent RNA polymerase

Virology
Y Hong, A G Hunt

Abstract

We have expressed the putative RNA-dependent RNA polymerase encoded by the potyvirus tobacco vein mottling virus (TVMV) in Escherichia coli as a glutathione S-transferase fusion protein. As prepared, the fusion protein possessed the poly(U) polymerase activity that is a hallmark of other picornavirus-encoded polymerases. In addition, this protein was able to utilize full-length TVMV RNA as a template for RNA synthesis. A fusion protein containing a mutation in the highly conserved GDD motif of the polymerase (GDD-->ADD) possessed 7% of the activity of the wild type. Our results confirm that the presumed polymerase encoded by TVMV is in fact an RNA-dependent RNA polymerase and that the GDD motif so widely seen in viral polymerases has an important function in the TVMV protein.

Citations

Aug 17, 2001·Journal of Virology·K Hirashima, Y Watanabe
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·S L AlamR F Gesteland
Feb 28, 2001·Virus Research·S Urcuqui-InchimaF Bernardi
Jan 22, 2003·Biochemical and Biophysical Research Communications·Olga EstebanMariano Cambra
Mar 30, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K IshiiT Miyamura
Oct 10, 2013·Molecular Plant Pathology·Juan Antonio GarcíaThierry Candresse
May 23, 2001·The Journal of General Virology·E SadowyM D Hulanicka
Apr 12, 2014·The Journal of General Virology·K I IvanovK Mäkinen
Jul 4, 2015·Macromolecular Rapid Communications·Ana Sanchez-SanchezJosé A Pomposo
Dec 30, 2014·Micron : the International Research and Review Journal for Microscopy·Katarzyna OtulakGrażyna Garbaczewska
Nov 18, 2015·Molecular Plant Pathology·Andres LõhmusKristiina Mäkinen
Sep 30, 2016·Frontiers in Genetics·Muhammad S IqbalIdrees A Nasir
Feb 26, 2019·Plant Biotechnology Journal·Xiaohui ZhanJiang Zhang
Sep 1, 2017·The Journal of General Virology·Ingrida OlendraiteAndrew E Firth
Jan 25, 2020·Molecular Plant Pathology·Kensaku MaejimaYasuyuki Yamaji
Feb 13, 2020·Plants·Kristin WidyasariKook-Hyung Kim
Jun 26, 2004·The Journal of Biological Chemistry·Pietri Puustinen, Kristiina Mäkinen
Nov 7, 2019·Virus Genes·Francisco Mosquera-YuquiFrancisco J Flores

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