RNA polymerase II 140wimp mutant and mutations in the TFIIH subunit XPB differentially affect homeotic gene expression in Drosophila

Genesis : the Journal of Genetics and Development
Luis GutiérrezMario Zurita

Abstract

Mutations in the XPB and XPD helicases of the DNA repair/transcription factor TFIIH are involved in several human genetic disorders. An unanswered problem concerning the complexity of the phenotype-genotype relationship is why mutations in individual subunits of TFIIH produce specific phenotypes and not many others. In order to investigate this question we tested whether mutations in the Drosophila XPB homolog, haywire (hay), would modify homeotic derepression phenotypes. In this work, we report that mutations in hay and in the 140-kDa subunit of the RNA polymerase II (RpII140wimp) act as dominant modifiers of the derepression phenotypes of the Sex combs reduced (Scr) and Ultrabithorax (Ubx) genes. The hay mutations only weakly suppress the Scr derepression phenotype caused by the Antp(Scx) mutation but not by Polycomb. In contrast, the RpII140wimp mutation strongly suppresses both Scr derepression phenotypes. In addition, the RpII140wimp also generates phenotypes indicative of loss of Ubx function. On the other hand, all the derepression homeotic phenotypes are sensitive to the generalized reduction of transcription levels when the flies are grown with actinomycin D. We also show that different promoter control regions have di...Continue Reading

References

Jan 1, 1992·American Journal of Medical Genetics·M A Nance, S A Berry
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R GangulyR Krishnan
Sep 1, 1990·Genetics·M A González-GaitánA García-Bellido
May 1, 1990·Journal of the American Academy of Dermatology·P H Itin, M R Pittelkow
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M A Mortin
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J A Kennison, J W Tamkun
Feb 1, 1996·Biochemical Genetics·L P BurkeM A Mortin
Jun 19, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A R Lehmann
Aug 15, 2000·Trends in Neurosciences·R L Rolig, P J McKinnon
Mar 15, 2002·Current Opinion in Genetics & Development·Jeffrey A Simon, John W Tamkun
Jun 20, 2002·Genetics·Jeffrey W Southworth, James A Kennison
Aug 27, 2002·Current Biology : CB·Alan Lehmann
Sep 11, 2002·Molecular Biology of the Cell·Carlos MerinoMario Zurita
Oct 24, 2002·Human Molecular Genetics·Elena BottaMiria Stefanini
Oct 11, 2003·Trends in Genetics : TIG·Mario Zurita, Carlos Merino
Jun 29, 2004·Nature Genetics·Jeffrey A RanishRuedi Aebersold

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