DOI: 10.1101/510156Jan 16, 2019Paper

RNA Polymerase II is involved in 18S and 25S ribosomal RNA transcription, in Candida albicans

BioRxiv : the Preprint Server for Biology
Jacob FleischmannPeter V Hauser

Abstract

Three different RNA polymerases are involved in ribosome production. Current understanding is that RNA Polymerase I (Pol I), under the control of TOR kinase, transcribes the large (25S) and the small (18S and 5.8S) subunits in a polycistronic fashion as a single unit. Recently we found 18S and 25S molecules resisting digestion by a 5'-phosphate-dependent exonuclease in C. albicans as they go into stationary phase, indicating that these molecules were different from the usual processed rRNA transcripts. By utilizing an anti-7-methylguanosine cap specific antibody and cap-binding protein, here we show that C. albicans in stationary growth phase, or when inhibited by rapamycin, produces 18S and 25S molecules with 5'-caps, which would make them resistant to 5'-phosphate-dependent exonuclease digestion We found the 5'-end of these molecules to be at the reported processing site both for 18S and 25S. We also show them to be polyadenylated. These two features are typical for products of RNA polymerase II (Pol II). We confirmed such a role for this enzyme by chromatin immunoprecipitation (ChIP), showing presence of Pol II on ribosomal RNA gene (rDNA) 18S and 25S sequences. Also with immuno-fluorescence microscopy, we show directly the ...Continue Reading

Related Concepts

Candida albicans
Cell Nucleolus
Digestion
Microscopy, Fluorescence
Poly A
Ribosomes
DNA-Directed RNA Polymerase
RNA, Messenger
Ribosomal RNA
Genes, rRNA

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