RNA polymerase III transcription from the human U6 and adenovirus type 2 VAI promoters has different requirements for human BRF, a subunit of human TFIIIB.

Molecular and Cellular Biology
R MitalN Hernandez

Abstract

Mammalian TFIIIB can be separated into two fractions required for transcription of the adenovirus type 2 VAI gene, which have been designated 0.38M-TFIIIB and 0.48M-TFIIIB. While 0.48M-TFIIIB has not been characterized, 0.38M-TFIIIB corresponds to a TBP-containing complex. We describe here the purification of this complex, which consists of TBP and a closely associated polypeptide of 88 kDa, and the isolation of a cDNA corresponding to the 88-kDa polypeptide. The predicted protein sequence reveals that the 88-kDa polypeptide corresponds to a human homolog of the Saccharomyces cerevisiae BRF protein, a subunit of yeast TFIIIB. Human BRF (hBRF) probably corresponds to TFIIIB90, a protein previously cloned by Wang and Roeder (Proc. Natl. Acad. Sci. USA 92:7026-7030, 1995), although its predicted amino acid sequence differs from that reported for TFIIIB90 over a stretch of 67 amino acids as a result of frameshifts. Immunodepletion of more than 90 to 95% of the hBRF present in a transcription extract severely debilitates transcription from the tRNA-type VAI promoter but does not affect transcription from the TATA box-containing human U6 promoter, suggesting that the 0.38M-TFIIIB complex, and perhaps hBRF as well, is not required for...Continue Reading

References

Oct 1, 1992·Genes & Development·T Colbert, S Hahn
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G A KassavetisE P Geiduschek
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S K YoshinagaA J Berk
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·G A KassavetisM Pisano
Aug 25, 1994·Nucleic Acids Research·J HuetA Sentenac
Dec 1, 1993·Molecular and Cellular Biology·R E Meyers, P A Sharp
Dec 16, 1993·Nature·Y XiongD Beach
Jul 1, 1993·Genes & Development·N Hernandez
Oct 20, 1993·Journal of Molecular Biology·A F BurnolA Sentenac
May 1, 1993·Molecular and Cellular Biology·J B EschenlauerD A Brow

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Citations

Oct 17, 1998·DNA Sequence : the Journal of DNA Sequencing and Mapping·C G Larminie, R J White
Jul 13, 2007·Biochemical and Biophysical Research Communications·Joby JacobLaura Schramm
Nov 25, 2003·The EMBO Journal·David C ArthurJ N Mark Glover
Jun 23, 2001·Journal of Molecular Biology·E P Geiduschek, G A Kassavetis
Aug 14, 2008·BMC Molecular Biology·Stephanie CabarcasLaura Schramm
Feb 19, 2004·Advances in Protein Chemistry·Robyn D Moir, Ian M Willis
Aug 2, 2000·The EMBO Journal·V McCullochS M Lobo-Ruppert
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·R W HenryN Hernandez
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·A KumarE P Geiduschek
Feb 27, 1999·Journal of Molecular Biology·S J McBryantJ M Gottesfeld
Oct 1, 2003·Archives of Insect Biochemistry and Physiology·M Juanita Martinez, Karen U Sprague
Jan 26, 2002·Molecular Biology of the Cell·Danyang ChenSui Huang
May 23, 2018·Genes & Development·Oleksandr DergaiNouria Hernandez
May 23, 2002·The Journal of Biological Chemistry·Pavel Cabart, Shona Murphy
Mar 18, 2006·Biological Chemistry·Frank Schoenen, Brunhilde Wirth
Mar 23, 2006·The Journal of Biological Chemistry·George A KassavetisE Peter Geiduschek
Jun 8, 2001·The Journal of Biological Chemistry·N Hernandez
Jun 27, 1998·The Journal of Biological Chemistry·H M Alzuherri, R J White
Dec 14, 2001·The Journal of Biological Chemistry·Wolfgang MeissnerKlaus H Seifart
Dec 10, 2002·The Journal of Biological Chemistry·Edward E SchmidtMario R Capecchi
Oct 23, 2002·Molecular and Cellular Biology·Beicong Ma, Nouria Hernandez
Nov 30, 2000·Molecular and Cellular Biology·J E SutcliffeR J White
Jan 30, 2004·In Vitro Cellular & Developmental Biology. Animal·Timothy E Shannon, Calvin B L James
Jun 22, 1999·Molecular and Cellular Biology·C G LarminieR J White
Apr 3, 2001·The Journal of Biological Chemistry·K Eichhorn, S P Jackson
Sep 15, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ying-Qi WangQing-Sheng Li

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