PMID: 7035823Jan 1, 1981Paper

RNA polymerase is required for DNA initiation in vitro

Molecular & General Genetics : MGG
S J Projan, J A Wechsler

Abstract

We have previously reported in vitro complementation assays for chromosome initiation that enable dnaA and dnaC mutant extracts to synthesize DNA. To examine the role of RNA polymerase in chromosome initiation, inhibitors of the enzyme and anti-RNA polymerase antibody were used. Though rifampicin failed to efficiently inhibit ribonucleoside triphosphate polymerization under the assay conditions, both streptolydigin and anti-RNA polymerase antibody abolished ribonucleic acid synthesis completely. Antibody effectively inhibited chromosome initiation in the dnaA mutant based reaction but streptolydigin did not. Neither streptolydigin nor antibody affected the dnaC-dependent assay. It was concluded that RNA polymerase is required for initiation but not necessarily to polymerize a polyribonucleotide. A scheme for the sequence of initiation events is presented.

References

Feb 1, 1978·Journal of Bacteriology·F C Kung, D A Glaser
Jan 28, 1972·Journal of Molecular Biology·H SchallerF Bonhoeffer
Feb 25, 1973·Journal of Molecular Biology·W H SchubachC I Davern
Jan 1, 1970·Molecular & General Genetics : MGG·P L Carl
Jan 1, 1971·Molecular & General Genetics : MGG·J A Wechsler, J D Gross
Feb 1, 1981·Journal of Bacteriology·S J Projan, J A Wechsler
Jan 1, 1981·Molecular & General Genetics : MGG·S J Projan, J A Wechsler

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Citations

Mar 1, 1986·Archives of Microbiology·K T Hiriyanna, T Ramakrishnan
Apr 25, 2007·Psychological Medicine·Mark A LauZindel V Segal
Jan 1, 1993·Journal of Assisted Reproduction and Genetics·N R VarneyK Franzen

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