RNA primer-primase complexes serve as the signal for polymerase recycling and Okazaki fragment initiation in T4 phage DNA replication

Proceedings of the National Academy of Sciences of the United States of America
Michelle M SpieringS J Benkovic

Abstract

The opposite strand polarity of duplex DNA necessitates that the leading strand is replicated continuously whereas the lagging strand is replicated in discrete segments known as Okazaki fragments. The lagging-strand polymerase sometimes recycles to begin the synthesis of a new Okazaki fragment before finishing the previous fragment, creating a gap between the Okazaki fragments. The mechanism and signal that initiate this behavior-that is, the signaling mechanism-have not been definitively identified. We examined the role of RNA primer-primase complexes left on the lagging ssDNA from primer synthesis in initiating early lagging-strand polymerase recycling. We show for the T4 bacteriophage DNA replication system that primer-primase complexes have a residence time similar to the timescale of Okazaki fragment synthesis and the ability to block a holoenzyme synthesizing DNA and stimulate the dissociation of the holoenzyme to trigger polymerase recycling. The collision with primer-primase complexes triggering the early termination of Okazaki fragment synthesis has distinct advantages over those previously proposed because this signal requires no transmission to the lagging-strand polymerase through protein or DNA interactions, the me...Continue Reading

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Citations

Oct 4, 2017·The Journal of Biological Chemistry·Stephen J Benkovic, Michelle M Spiering
May 1, 2021·Frontiers in Microbiology·Jan BergschGeorg Lipps
Jun 22, 2021·Annual Review of Biochemistry·Stephen J Benkovic
Sep 8, 2017·Chemical Research in Toxicology·Michael A TrakselisAurea M Chu

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