RNA recognition by the embryonic cell fate determinant and germline totipotency factor MEX-3.

Proceedings of the National Academy of Sciences of the United States of America
John M PaganoSean P Ryder

Abstract

Totipotent stem cells have the potential to differentiate into every cell type. Renewal of totipotent stem cells in the germline and cellular differentiation during early embryogenesis rely upon posttranscriptional regulatory mechanisms. The Caenorhabditis elegans RNA binding protein, MEX-3, plays a key role in both processes. MEX-3 is a maternally-supplied factor that controls the RNA metabolism of transcripts encoding critical cell fate determinants. However, the nucleotide sequence specificity and requirements of MEX-3 mRNA recognition remain unclear. Only a few candidate regulatory targets have been identified, and the full extent of the network of MEX-3 targets is not known. Here, we define the consensus sequence required for MEX-3 RNA recognition and demonstrate that this element is required for MEX-3 dependent regulation of gene expression in live worms. Based on this work, we identify several candidate MEX-3 targets that help explain its dual role in regulating germline stem cell totipotency and embryonic cell fate specification.

References

Aug 30, 1990·Nature·A D Ellington, J W Szostak
Jan 20, 1998·Trends in Genetics : TIG·M DsouzaR Overbeek
May 11, 2002·RNA·Aleksandr V Makeyev, Stephen A Liebhaber
Jan 14, 2004·Nature Structural & Molecular Biology·Sean P RyderJames R Williamson
Feb 11, 2004·The Journal of Biological Chemistry·Martino DonniniNicola Schiavone
Feb 28, 2006·Developmental Biology·Ingrid D'AgostinoKuppuswamy Subramaniam
Sep 22, 2006·Methods in Molecular Biology·Vida Praitis
Nov 30, 2006·Cell·Geraldine Seydoux, Robert E Braun
Jan 12, 2007·Development Genes and Evolution·Molly JudJennifer A Schisa
Feb 1, 2007·The Journal of Biological Chemistry·John M PaganoSean P Ryder
Mar 28, 2008·Critical Reviews in Biochemistry and Molecular Biology·Brian M Farley, Sean P Ryder
Apr 22, 2008·The FEBS Journal·Roberto ValverdeLynne Regan
Aug 12, 2008·Developmental Biology·Christopher M GalloGeraldine Seydoux
Sep 27, 2008·Current Biology : CB·Christopher MerrittGeraldine Seydoux
Oct 28, 2008·RNA·Brian M FarleySean P Ryder
Oct 28, 2008·Nature Genetics·Christian Frøkjaer-JensenErik M Jorgensen
Mar 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Fionna E LoughlinJoel P Mackay

❮ Previous
Next ❯

Citations

Sep 4, 2013·Trends in Biochemical Sciences·Bruno PereiraRaquel Almeida
Feb 24, 2012·Molecular Biology of the Cell·Yan JiaoBaisong Lu
Oct 5, 2012·Molecular Biology of the Cell·Brian M Farley, Sean P Ryder
Jan 24, 2014·PLoS Genetics·John M PaganoJohn T Lis
Jan 7, 2016·Frontiers in Molecular Biosciences·Patrícia Shigunov, Bruno Dallagiovanna
Feb 23, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Natalia V KirienkoDavid S Fay
Oct 5, 2011·Developmental Biology·Rana MainpalKuppuswamy Subramaniam
Jun 14, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ebru KaymakSean P Ryder
Mar 21, 2019·Development·Shaohe WangRebecca A Green
Sep 10, 2013·The Journal of Biological Chemistry·Ebru Kaymak, Sean P Ryder
Feb 8, 2013·G3 : Genes - Genomes - Genetics·Alex M TamburinoAlbertha J M Walhout
Nov 7, 2019·Genetics·Joel Rothman, Sophie Jarriault
Jul 14, 2017·Translation·Alex M TamburinoAlbertha J M Walhout
Nov 1, 2016·Genes & Development·Deni SubasicMichael O Hengartner
Oct 18, 2013·Development·Marieke OldenbroekRueyling Lin
Jun 3, 2021·Biology·Marcell LedererStefan Hüttelmaier

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.