RNA Seq analysis of the Eimeria tenella gametocyte transcriptome reveals clues about the molecular basis for sexual reproduction and oocyst biogenesis

BMC Genomics
Robert A WalkerNicholas C Smith

Abstract

The protozoan Eimeria tenella is a common parasite of chickens, causing avian coccidiosis, a disease of on-going concern to agricultural industries. The high prevalence of E. tenella can be attributed to the resilient oocyst stage, which is transmitted between hosts in the environment. As in related Coccidia, development of the eimerian oocyst appears to be dependent on completion of the parasite's sexual cycle. RNA Seq transcriptome profiling offers insights into the mechanisms governing the biology of E. tenella sexual stages (gametocytes) and the potential to identify targets for blocking parasite transmission. Comparisons between the sequenced transcriptomes of E. tenella gametocytes and two asexual developmental stages, merozoites and sporozoites, revealed upregulated gametocyte transcription of 863 genes. Many of these genes code for proteins involved in coccidian sexual biology, such as oocyst wall biosynthesis and fertilisation, and some of these were characterised in more depth. Thus, macrogametocyte-specific expression and localisation was confirmed for two proteins destined for incorporation into the oocyst wall, as well as for a subtilisin protease and an oxidoreductase. Homologues of an oocyst wall protein and oxid...Continue Reading

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Citations

Jan 29, 2016·Journal of Invertebrate Pathology·Abul Farah Md HasanuzzamanPaulino Martínez
Mar 5, 2016·Parasites & Vectors·Robert A WalkerNicholas C Smith
Dec 15, 2015·Frontiers in Veterinary Science·Aruna ShresthaAnja Joachim
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Datasets Mentioned

BETA
AB723702.1

Methods Mentioned

BETA
transcription profiling
RNA Seq
electrophoresis
light microscopy
PCR
glycosylation
confocal microscopy
RNA-Seq
ELISA

Software Mentioned

ClustalW
Bioconductor
Blast2Go
SignalP
Huygens Essential
GraphPad
Leica Confocal
ToxoDB
DESeq
Leica LAS AF

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