RNA-Seq Analysis of the Expression of Genes Encoding Cell Wall Degrading Enzymes during Infection of Lupin (Lupinus angustifolius) by Phytophthora parasitica

PloS One
Leila M BlackmanAdrienne R Hardham

Abstract

RNA-Seq analysis has shown that over 60% (12,962) of the predicted transcripts in the Phytophthora parasitica genome are expressed during the first 60 h of lupin root infection. The infection transcriptomes included 278 of the 431 genes encoding P. parasitica cell wall degrading enzymes. The transcriptome data provide strong evidence of global transcriptional cascades of genes whose encoded proteins target the main categories of plant cell wall components. A major cohort of pectinases is predominantly expressed early but as infection progresses, the transcriptome becomes increasingly dominated by transcripts encoding cellulases, hemicellulases, β-1,3-glucanases and glycoproteins. The most highly expressed P. parasitica carbohydrate active enzyme gene contains two CBM1 cellulose binding modules and no catalytic domains. The top 200 differentially expressed genes include β-1,4-glucosidases, β-1,4-glucanases, β-1,4-galactanases, a β-1,3-glucanase, an α-1,4-polygalacturonase, a pectin deacetylase and a pectin methylesterase. Detailed analysis of gene expression profiles provides clues as to the order in which linkages within the complex carbohydrates may come under attack. The gene expression profiles suggest that (i) demethylation...Continue Reading

References

Aug 28, 1998·Microbiology·Gary WilliamsonCRaig B Faulds
Mar 27, 1999·European Journal of Biochemistry·J A BenenJ Visser
Jul 7, 2000·Fungal Genetics and Biology : FG & B·C Cano-CancholaC A Leal-Morales
Aug 10, 2000·Proceedings of the National Academy of Sciences of the United States of America·L M RogersP E Kolattukudy
Feb 27, 2001·Journal of Bacteriology·J C Hurlbert, J F Preston
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·R P de Vries, J Visser
Mar 23, 2002·Phytochemistry·Frédéric BrunnerThorsten Nürnberger
Jan 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Janne LehtiöTuula T Teeri
Mar 7, 2003·Fungal Genetics and Biology : FG & B·Adele McLeodWilliam E Fry
Jun 25, 2004·The Biochemical Journal·Alisdair B BorastonGideon J Davies
Jul 17, 2004·Microbiology·David J Adams
Sep 21, 2004·Annual Review of Plant Biology·Malcolm A O'NeillAlan G Darvill
Dec 25, 2004·Science·Chris SomervilleHeather Youngs
Nov 2, 2005·Nature Reviews. Molecular Cell Biology·Daniel J Cosgrove
Jan 13, 2006·Molecular Plant-microbe Interactions : MPMI·Nélida BritoCeledonio González
Jan 5, 2007·Annual Review of Plant Biology·Georg J Seifert, Keith Roberts
Mar 14, 2007·Annual Review of Phytopathology·Ralph Hückelhoven
Oct 24, 2007·Applied Microbiology and Biotechnology·Barend J M de WetBernard A Prior
Feb 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Maura C CannonMarcia J Kieliszewski
Mar 7, 2008·Molecular Plant-microbe Interactions : MPMI·Chih-Hang WuRuey-Fen Liou
May 24, 2008·BMC Plant Biology·Susan E MarcusJ Paul Knox
Oct 8, 2008·Nucleic Acids Research·Brandi L CantarelBernard Henrissat
Feb 18, 2009·Molecular Genetics and Genomics : MGG·Pamela Hui Peng GanAdrienne R Hardham
Jul 21, 2009·Carbohydrate Research·Kerry Hosmer Caffall, Debra Mohnen
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Chaoguang TianN Louise Glass
Feb 25, 2010·Biotechnology for Biofuels·Valdeir Arantes, Jack N Saddler
Apr 3, 2010·Advances in Applied Microbiology·Carl J YeomanIsaac K O Cann

❮ Previous
Next ❯

Citations

Feb 24, 2016·Insect Biochemistry and Molecular Biology·Roy KirschYannick Pauchet
Jul 26, 2016·Frontiers in Plant Science·Tom M Raaymakers, Guido Van den Ackerveken
Dec 14, 2017·Nature Communications·Jie HuangSuomeng Dong
Apr 3, 2020·Molecular Plant-microbe Interactions : MPMI·Marco ThinesDavid J Studholme
May 13, 2020·The New Phytologist·Petra C BoevinkStephen C Whisson
Jul 28, 2019·Nature Reviews. Microbiology·Madhav P ThakurStefan Geisen
May 19, 2017·Molecular Plant Pathology·Adrienne R Hardham, Leila M Blackman
May 16, 2017·Annual Review of Microbiology·Howard S Judelson
Aug 10, 2017·The Science of the Total Environment·Melline Fontes NoronhaValéria Maia de Oliveira
Jun 6, 2020·Current Biology : CB·Hassan SalemNicole M Gerardo
Jan 22, 2021·Journal of Experimental Botany·Martin SolanskýJan Lochman
Aug 14, 2021·Science·Federico SabbadinSimon J McQueen-Mason

❮ Previous
Next ❯

Datasets Mentioned

BETA
DQ241759
SRP061812

Methods Mentioned

BETA
RNA-Seq
PCR
Genome Sequencing

Software Mentioned

BioKanga
DESeq
RotorGene
R
RNASeq
FastQC
Illumina Consensus Assessment of Sequence and Variation ( CASA...
CAZyme

Related Concepts

Related Feeds

Biosynthetic Transformations

Biosyntheic transformtions are multi-step, enzyme-catalyzed processes where substrates are converted into more complex products in living organisms. Simple compounds are modified, converted into other compounds, or joined together to form macromolecules. Discover the latest research on biosynthetic transformations here.