Dec 13, 2015

RNA-seq and Bulked Segregant Analysis of a Gene Related to High Growth in Ginkgo biloba Half-siblings.

BioRxiv : the Preprint Server for Biology
Hai Xia TangLi Min Sun

Abstract

We combined mRNA sequencing (RNA-Seq) with bulked segregant analysis (BSA) to fine map significant agronomic trait genes by developing polymorphism molecular markers at the transcriptome level. RNA-Seq data provides BSA with genotype information in RNA Pool to screen out linked genes (low in false positives) after data analysis, and the efficiency of development and verification of the linked polymorphism marker is greatly improved. This combined approach (named BSR) has been applied to plant transcriptome sequencing in sunflower, corn, wheat, and Arabidopsis thaliana. Ginkgo biloba is a deciduous tree that belongings to the Ginkgoaceae family and has been recognized as a “living fossil”. It has important economic and cultural value in China. The lifetime of G. biloba is very long, and its growth is relatively slow. Studies have shown that geographic latitude, temperature, and soil nutrient influence the growth of G. biloba; however, little is known about growth-related genes. In this study, transcriptome sequencing of high growth (GD) and low growth (BD) samples of G. biloba half-sib families was performed. After assembling the clean reads, 601 differential expression genes were detected and 513 of them were assigned functiona...Continue Reading

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Mentioned in this Paper

Biological Markers
Gene Polymorphism
Guanosine
Study
Ginkgo biloba Whole
Genes
Sequence Determinations, RNA
Helianthus species
Arsenophonus symbiont of Ornithomya biloba
Corn of Toe

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