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RNA-seq mixology: designing realistic control experiments to compare protocols and analysis methods

Nucleic Acids Research

Dec 3, 2016

Aliaksei Z HolikMatthew E Ritchie

Abstract

Carefully designed control experiments provide a gold standard for benchmarking different genomics research tools. A shortcoming of many gene expression control studies is that replication involves profiling the same reference RNA sample multiple times. This leads to low, pure technical...read more

Mentioned in this Paper

Study
Small Nuclear RNA
RNA, Untranslated
Sequence Determinations, RNA
Virus Replication
Cell Culture Techniques
Profile (Lab Procedure)
Gene Expression
Poly A
Small Nucleolar RNA Location
51
1
83
Paper Details
References
  • References51
  • Citations5
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  • References51
  • Citations5

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RNA-seq mixology: designing realistic control experiments to compare protocols and analysis methods

Nucleic Acids Research

Dec 3, 2016

Aliaksei Z HolikMatthew E Ritchie

PMID: 27899618

DOI: 10.1093/nar/gkw1063

Abstract

Carefully designed control experiments provide a gold standard for benchmarking different genomics research tools. A shortcoming of many gene expression control studies is that replication involves profiling the same reference RNA sample multiple times. This leads to low, pure technical...read more

Mentioned in this Paper

Study
Small Nuclear RNA
RNA, Untranslated
Sequence Determinations, RNA
Virus Replication
Cell Culture Techniques
Profile (Lab Procedure)
Gene Expression
Poly A
Small Nucleolar RNA Location
51
1
83

Feeds With Similar Papers

RNA Splicing (Keystone)

The majority of human genes that encode proteins undergo alternative pre-mRNA splicing and mutations that affect splicing are more prevalent than previously thought. Discover the latest research on RNA splicing and co-transcriptional splicing in this feed.

RNA Splicing

The majority of human genes that encode proteins undergo alternative pre-mRNA splicing and mutations that affect splicing are more prevalent than previously thought. Discover the latest research on RNA Splicing here.

Related Papers

Genome Biology

From RNA-seq reads to differential expression results

Genome BiologyDecember 24, 2010
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Estimating differential expression from multiple indicators

Nucleic Acids ResearchMarch 4, 2014
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Paper Details
References
  • References51
  • Citations5
12345...
  • References51
  • Citations5

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