RNA-seq Profiles of Immune Related Genes in the Spleen of Necrotic Enteritis-afflicted Chicken Lines

Asian-Australasian Journal of Animal Sciences
Anh Duc TruongH S Lillehoj

Abstract

The study aimed to compare the necrotic enteritis (NE)-induced transcriptome differences between the spleens of Marek's disease resistant chicken line 6.3 and susceptible line 7.2 co-infected with Eimeria maxima/Clostridium perfringens using RNA-Seq. Total RNA from the spleens of two chicken lines were used to make libraries, generating 42,736,296 and 42,617,720 usable reads, which were assembled into groups of 29,897 and 29,833 mRNA genes, respectively. The transcriptome changes were investigated using the differentially expressed genes (DEGs) package, which indicated 3,255, 2,468 and 2,234 DEGs of line 6.3, line 7.2, and comparison between two lines, respectively (fold change ≥2, p<0.01). The transcription levels of 14 genes identified were further examined using qRT-PCR. The results of qRT-PCR were consistent with the RNA-seq data. All of the DEGs were analysed using gene ontology terms, the Kyoto Encyclopedia of Genes and Genomes (KEGG) database and the DEGs in each term were found to be more highly expressed in line 6.3 than in line 7.2. RNA-seq analysis indicated 139 immune related genes, 44 CD molecular genes and 150 cytokines genes which were differentially expressed among chicken lines 6.3 and 7.2 (fold change ≥2, p<0....Continue Reading

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Citations

Jan 24, 2017·Asian-Australasian Journal of Animal Sciences·Deivendran RengarajYeong Ho Hong
Jun 28, 2019·Probiotics and Antimicrobial Proteins·Xiaoxiao GuDongmei Zhang
Jun 11, 2019·Oxidative Medicine and Cellular Longevity·Shicheng BiSonghua Hu
Apr 25, 2017·The Journal of Poultry Science·Anh Duc TruongHyun S Lillehoj
Aug 18, 2020·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Sayantan SurVinod Kumar
Jan 9, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Jinxin LiuLi Wang
Nov 6, 2020·Asian-Australasian Journal of Animal Sciences·Gi Ppeum HanDong Yong Kil

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Methods Mentioned

BETA
RNA-Seq
PCR

Software Mentioned

Cluster
PALMapper
Qpalma
Cufflinks
Java Treeview
Splicemap
Lasergene
DAVID Bioinformatics Resources
GSNAP
Blast2GO

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