RNA-Seq Revealed Expression of Many Novel Genes Associated With Leishmania donovani Persistence and Clearance in the Host Macrophage

Frontiers in Cellular and Infection Microbiology
Mohammad ShadabNahid Ali

Abstract

Host- as well as parasite-specific factors are equally crucial in allowing either the Leishmania parasites to dominate, or host macrophages to resist infection. To identify such factors, we infected murine peritoneal macrophages with either the virulent (vAG83) or the non-virulent (nvAG83) parasites of L. donovani. Then, through dual RNA-seq, we simultaneously elucidated the transcriptomic changes occurring both in the host and the parasites. Through Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the differentially expressed (DE) genes, we showed that the vAG83-infected macrophages exhibit biased anti-inflammatory responses compared to the macrophages infected with the nvAG83. Moreover, the vAG83-infected macrophages displayed suppression of many important cellular processes, including protein synthesis. Further, through protein-protein interaction study, we showed significant downregulation in the expression of many hubs and hub-bottleneck genes in macrophages infected with vAG83 as compared to nvAG83. Cell signaling study showed that these two parasites activated the MAPK and PI3K-AKT signaling pathways differentially in the host cells. Through gene ontology analyses of the parasite-specific genes, we disc...Continue Reading

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Citations

Apr 16, 2020·Parasite Immunology·Gabriela Pessenda, João Santana da Silva
Jul 3, 2020·Genes·Arijit BhattacharyaChristopher Fernandez-Prada
Feb 23, 2021·PLoS Neglected Tropical Diseases·Carlos M RestrepoPatricia L Fernández

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Methods Mentioned

BETA
RNA-Seq
FCS
Assay
cDNA library
PCR

Software Mentioned

GOseq
TopHat
Trimmomatic
GraphPad
Cytoscape
Hub Objects Analyzer Hubba
FastQC
R
in
DESeq

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