RNA-Seq Reveals Extensive Transcriptional Response to Heat Stress in the Stony Coral Galaxea fascicularis

Frontiers in Genetics
Jing HouYan Wang

Abstract

Galaxea fascicularis, a stony coral belonging to family Oculinidae, is widely distributed in Red Sea, the Gulf of Aden and large areas of the Indo-Pacific oceans. So far there is a lack of gene expression knowledge concerning this massive coral. In the present study, G. fascicularis was subjected to heat stress at 32.0 ± 0.5°C in the lab, we found that the density of symbiotic zooxanthellae decreased significantly; meanwhile apparent bleaching and tissue lysing were observed at 10 h and 18 h after heat stress. The transcriptome responses were investigated in the stony coral G. fascicularis during heat bleaching using RNA-seq. A total of 42,028 coral genes were assembled from over 439 million reads. Gene expressions were compared at 10 and 18 h after heat stress. The significantly upregulated genes found in the Control_10h vs. Heat_10h comparison, presented mainly in GO terms related with DNA integration and unfolded protein response; and for the Control_18h vs. Heat_18h comparison, the GO terms include DNA integration. In addition, comparison between groups of Control_10h vs. Heat_10h and Control_18h vs. Heat_18h revealed that 125 genes were significantly upregulated in common between the two groups, whereas 21 genes were signi...Continue Reading

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Citations

Nov 7, 2019·Global Change Biology·Homère J Alves MonteiroJérémy Le Luyer
Aug 11, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Madeleine J H van Oppen, Mónica Medina
Dec 29, 2019·Trends in Genetics : TIG·Phillip A ClevesDebashish Bhattacharya
Mar 25, 2021·Trends in Ecology & Evolution·Matthew C YatesMelania E Cristescu

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Datasets Mentioned

BETA
SRP083089

Methods Mentioned

BETA
PCR
PCA
protein folding
RNA-seq
acylation
acetylation

Software Mentioned

BiNGO
Trinotate
Trinity
Cytoscape
TransDecoder
BLASTx
BLAST
CummeRbund
Cufflinks
BLASTP

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