RNA-Seq Reveals OTA-Related Gene Transcriptional Changes in Aspergillus carbonarius

PloS One
Donato GerinFrancesco Faretra

Abstract

Ochratoxin A (OTA) is a mycotoxin harmful for animals and humans. Aspergillus carbonarius is the main responsible for OTA contamination of grapes and derived products. Gene transcriptional profiling of 4 A. carbonarius strains was carried out by RNA-Seq analysis to study transcriptome changes associated with OTA production. By comparing OTA inducing (OTAI) vs. non-inducing (OTAN) cultural conditions, a total of 3,705 differentially expressed genes (DEGs) (fold change > |2| and FDR ≤ 0.05) were identified. Several genes involved in primary metabolic processes, with particular regard to carbohydrate and amino acid metabolisms, secondary metabolic processes, transport, response to stress and sporulation were up-regulated by OTAI conditions at all the analysed sampling times (4, 6 and 8 DAI) or starting from 6 DAI. Highly up-regulated DEGs encoding enzymes involved in biosynthesis of secondary metabolites, oxidoreductases, transporters and transcription factors were examined for their potential involvement in OTA biosynthesis and related metabolic pathways. Differential expression of genes encoding polyketide synthases (pks), non-ribosomal peptide synthetases (nrps) and chloroperoxidase (cpo) was validated by RT-qPCR. Among cluster...Continue Reading

References

Dec 1, 1992·Journal of Animal Science·R R Marquardt, A A Frohlich
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·D W BrownT J Leonard
Aug 1, 1996·Food Additives and Contaminants·B Zimmerli, R Dick
Apr 1, 1997·Fungal Genetics and Biology : FG & B·D Guzmán-de-Peña, J Ruiz-Herrera
Sep 15, 1999·Molecular & General Genetics : MGG·N J AlexanderT M Hohn
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Ana M CalvoNancy P Keller
Oct 10, 2002·International Journal of Food Microbiology·F J CabañesA Pons
Jul 25, 2003·International Journal of Food Microbiology·Sima YaronKarl R Matthews
Jan 1, 1965·American Journal of Botany·K E SANDERSON, A M SRB
Mar 10, 2004·Applied and Environmental Microbiology·Jiujiang YuJoan W Bennett
Mar 7, 2006·Fungal Genetics and Biology : FG & B·John O'CallaghanAlan D W Dobson
Aug 25, 2007·International Journal of Food Microbiology·Rufino MateoMisericordia Jiménez
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Aug 23, 2008·International Journal of Food Microbiology·Alessandro BottonAngelo Ramina
Oct 29, 2008·Fungal Genetics and Biology : FG & B·Nafees BachaAhmed Lebrihi
Nov 29, 2008·International Journal of Food Microbiology·Antonia GalloGiuseppina Mulè
Sep 1, 2009·Annual Review of Genomics and Human Genetics·Olena MorozovaMarco A Marra
Oct 24, 2009·Fungal Genetics and Biology : FG & B·Masahiro OgawaYasuji Koyama
May 4, 2010·Fungal Genetics and Biology : FG & B·Axel A Brakhage, Volker Schroeckh
Jul 27, 2010·International Journal of Food Microbiology·A Crespo-SempereP V Martínez-Culebras
May 3, 2011·International Journal of Food Microbiology·A Crespo-SempereP V Martínez-Culebras
Oct 7, 2011·Bioinformatics·Eleanor A HoweJohn Quackenbush
Apr 1, 2010·Toxins·André el Khoury, Ali Atoui
Mar 14, 2012·Applied Microbiology and Biotechnology·Massimo ReverberiCorrado Fanelli
Mar 2, 2013·Studies in Mycology·P KrijgsheldH A B Wösten
Jun 1, 2006·Mycotoxin Research·R GeisenA Karolewiez
Jun 6, 2013·Nucleic Acids Research·Kai BlinTilmann Weber
Sep 18, 2013·International Journal of Food Microbiology·A Crespo-SempereA J Ramos
Apr 5, 2014·International Journal of Food Microbiology·Antonia GalloGiancarlo Perrone

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Citations

Apr 5, 2018·Scientific Reports·Gemma CastelláF Javier Cabañes
Aug 8, 2021·International Journal of Molecular Sciences·Antonia Gallo, Giancarlo Perrone
May 9, 2019·Biochemistry·Sebastian Obermaier, Michael Müller

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Datasets Mentioned

BETA
SRP059422

Methods Mentioned

BETA
RNA-Seq
protein folding
gene knockout

Software Mentioned

Primer3
ClusterBlast
BlastP
ArrayStar
OTAN
Blast2GO
Galaxy
AntiSMASH
FastX
CASAVA

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