RNA-seq transcriptional profiling of Leishmania amazonensis reveals an arginase-dependent gene expression regulation

PLoS Neglected Tropical Diseases
Juliana Ide AokiLucile Maria Floeter-Winter

Abstract

Leishmania is a protozoan parasite that alternates its life cycle between the sand-fly vector and the mammalian host. This alternation involves environmental changes and leads the parasite to dynamic modifications in morphology, metabolism, cellular signaling and regulation of gene expression to allow for a rapid adaptation to new conditions. The L-arginine pathway in L. amazonensis is important during the parasite life cycle and interferes in the establishment and maintenance of the infection in mammalian macrophages. Host arginase is an immune-regulatory enzyme that can reduce the production of nitric oxide by activated macrophages, directing the availability of L-arginine to the polyamine pathway, resulting in parasite replication. In this work, we performed transcriptional profiling to identify differentially expressed genes in L. amazonensis wild-type (La-WT) versus L. amazonensis arginase knockout (La-arg-) promastigotes and axenic amastigotes. A total of 8253 transcripts were identified in La-WT and La-arg- promastigotes and axenic amastigotes, about 60% of them codifying hypothetical proteins and 443 novel transcripts, which did not match any previously annotated genes. Our RNA-seq data revealed that 85% of genes were c...Continue Reading

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Citations

Jan 31, 2018·Frontiers in Microbiology·Sandra M MuxelLucile M Floeter-Winter
May 28, 2019·Journal of Enzyme Inhibition and Medicinal Chemistry·Andreza R GarciaAnderson S Pinheiro
Dec 15, 2019·International Journal of Molecular Sciences·Sandra Marcia MuxelCoral Barbas
Jan 8, 2020·Cells·Stephanie Maia AcuñaSandra Marcia Muxel
Feb 20, 2019·Non-coding RNA·Juliane C R FernandesSandra M Muxel
Oct 5, 2018·Frontiers in Cellular and Infection Microbiology·Patricia Sampaio Tavares VerasJuliana Perrone Bezerra de Menezes
Dec 18, 2018·Frontiers in Immunology·Sandra Marcia MuxelLucile Maria Floeter-Winter
Jul 16, 2019·Current Opinion in Microbiology·Juliana Ide AokiLucile Maria Floeter-Winter
Jul 3, 2021·International Journal of Molecular Sciences·Maricruz Mamani-HuancaÁngeles López-Gonzálvez

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Datasets Mentioned

BETA
PRJNA380128
SRX2661998
SRX2661999

Methods Mentioned

BETA
RNA-seq
PCR
protein folding

Software Mentioned

TopHat
FastQC
PikoReal
Trimmomatic
Cufflinks

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