RNA-seq transcriptional profiling of Herbaspirillum seropedicae colonizing wheat (Triticum aestivum) roots

Plant Molecular Biology
Vânia Cs PankieviczEmanuel M Souza

Abstract

Herbaspirillum seropedicae is a diazotrophic and endophytic bacterium that associates with economically important grasses promoting plant growth and increasing productivity. To identify genes related to bacterial ability to colonize plants, wheat seedlings growing hydroponically in Hoagland's medium were inoculated with H. seropedicae and incubated for 3 days. Total mRNA from the bacteria present in the root surface and in the plant medium were purified, depleted from rRNA and used for RNA-seq profiling. RT-qPCR analyses were conducted to confirm regulation of selected genes. Comparison of RNA profile of root attached and planktonic bacteria revealed extensive metabolic adaptations to the epiphytic life style. These adaptations include expression of specific adhesins and cell wall re-modeling to attach to the root. Additionally, the metabolism was adapted to the microxic environment and nitrogen-fixation genes were expressed. Polyhydroxybutyrate (PHB) synthesis was activated, and PHB granules were stored as observed by microscopy. Genes related to plant growth promotion, such as auxin production were expressed. Many ABC transporter genes were regulated in the bacteria attached to the roots. The results provide new insights into...Continue Reading

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Citations

Jan 6, 2019·Applied and Environmental Microbiology·Luis Paulo Silveira AlvesGary Stacey
Feb 11, 2020·BMC Genomics·Tatiane DobrzanskiMaria Berenice Reynaud Steffens
Jul 8, 2020·Applied and Environmental Microbiology·Luis Paulo Silveira AlvesMarcelo Müller-Santos
Apr 24, 2019·International Journal of Molecular Sciences·Artur PinskiRobert Hasterok
Jul 5, 2017·Plant Molecular Biology·Glaucio ValdameriEmanuel Maltempi de Souza
Sep 24, 2019·Molecular Plant-microbe Interactions : MPMI·Beverly J AgtucaGary Stacey
Jul 25, 2019·Scientific Reports·Liziane Cristina Campos Brusamarello-SantosEmanuel Maltempi de Souza
May 23, 2020·Genetics and Molecular Biology·Eliandro EspindulaLuciane Maria Pereira Passaglia
Aug 25, 2018·Frontiers in Microbiology·Mónica RosenbluethEsperanza Martínez-Romero
Oct 30, 2020·FEMS Microbiology Reviews·Marcelo Müller-SantosAnna Maria Pirttilä
Oct 24, 2020·Current Genomics·Minaxi SharmaPratishtha Gupta
Jan 5, 2021·World Journal of Microbiology & Biotechnology·Cecilia TauléFederico Battistoni
Jan 12, 2021·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Marine ValetteFlorence Wisniewski-Dyé
Oct 12, 2018·Cell Host & Microbe·Asaf LevyTanja Woyke
Dec 12, 2020·Saudi Journal of Biological Sciences·Ayomide Emmanuel Fadiji, Olubukola Oluranti Babalola
May 7, 2021·FEMS Microbiology Reviews·Marcelo Müller-SantosAnna Maria Pirttilä
Dec 17, 2020·MBio·Fernanda Plucani do AmaralGary Stacey
Jun 18, 2021·3 Biotech·Daniella Duarte Villarinho PessoaJean Luis Simoes-Araujo
Nov 21, 2021·Journal of Applied Microbiology·Om Parkash AhlawatGyanendra Singh

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