RNA sequence heterogeneity in natural populations of three satellite RNAs of cucumber mosaic virus.

Virology
G Kurath, P Palukaitis

Abstract

Sequence heterogeneity within populations of three satellite RNAs of cucumber mosaic virus (CMV) was assessed using two different approaches. Comparison of the nucleotide sequences of several cDNA clones of each satellite RNA revealed microsequence heterogeneity, which is often seen in populations of RNA genomes. RNase protection assays using minus-sense satellite RNA probes were used to detect major sites of heterogeneity within natural populations of each satellite RNA. In RNase protection assays of WL1-sat RNA populations, no major sites of heterogeneity were detected within seven different populations, including preparations from four different host plant species. In contrast, RNase protection assays of nine populations of B1-sat RNA showed three different patterns, which were most likely due to the existence of the B1-sat RNA populations as mixtures in which different sequence variants predominated in different preparations. Assays of five independent populations of D-sat RNA revealed a single major site of heterogeneity which was common to each population and was localized at approximately nucleotide 225 of the 335-nucleotide satellite sequence. This common site of heterogeneity is a feature of the D-sat RNA population st...Continue Reading

References

Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E WinterM Perucho
Nov 7, 1986·Cell·K Kirkegaard, D Baltimore
Oct 25, 1980·Journal of Molecular Biology·F SangerB A Roe
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P GoeletJ Karn
Mar 26, 1982·Science·J HollandS VandePol
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P AhlquistL S Loesch-Fries
Apr 22, 1977·Science·J M Kaper, H E Waterworth
Apr 15, 1981·Virology·H Donis-KellerJ M Clark

❮ Previous
Next ❯

Citations

Jan 1, 1995·Journal of Molecular Evolution·M J Roossinck, P Palukaitis
Oct 1, 1996·Nature Biotechnology·P Palukaitis, M J Roossinck
Mar 1, 2001·Molecular Plant Pathology·M J Roossinck
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D E Sleat, P Palukaitis
Oct 24, 2008·Phytopathology·O CabreraK B Scholthof
Jan 1, 1997·Annual Review of Phytopathology·M J Roossinck
Nov 10, 2001·Annual Review of Phytopathology·F García-ArenalJ M Malpica
Oct 20, 1991·Journal of Molecular Biology·A Fraile, F García-Arenal
Nov 13, 2009·The Journal of General Virology·Kazusato OhshimaAdrian J Gibbs
Jan 22, 2005·The Journal of General Virology·Zhongyang TanKazusato Ohshima
Jun 1, 1992·Microbiological Reviews·M J RoossinckP Palukaitis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.