RNA-sequencing-based comparative analysis of human hepatic progenitor cells and their niche from alcoholic steatohepatitis livers

Cell Death & Disease
An CeulemansTania Roskams

Abstract

Hepatic progenitor cells (HPCs) are small cells with a relative large oval nucleus and a scanty cytoplasm situated in the canals of Hering that express markers of (immature) hepatocytes and cholangiocytes. HPCs are present in large numbers in alcoholic steatohepatitis (ASH), one of the leading causes of chronic liver disease. To date, the mechanisms responsible for proliferation and differentiation of human HPCs are still poorly understood and the role of HPCs in ASH development is unknown. In this study, we aimed to characterise human HPCs and their interactions with other cells through comparison, on both protein and RNA level, of HPC-enriched cell populations from adult human liver tissue using different isolation methods. Fresh human liver tissue was collected from ASH explant livers and HPC-enriched cell populations were obtained via four different isolation methods: side population (SP), epithelial cell adhesion molecule (EpCAM) and trophoblast antigen 2 (TROP-2) membrane marker isolation and laser capture microdissection. Gene expression profiles of fluorescent-activated cell-sorted HPCs, whole liver extracts and laser microdissected HPC niches were determined by RNA-sequencing. Immunohistochemical evaluation of the isol...Continue Reading

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Citations

Sep 25, 2019·Cells·Stefaan VerhulstLeo A van Grunsven
Jan 3, 2019·Hepatology International·Beatriz Aguilar-Bravo, Pau Sancho-Bru
Mar 7, 2020·Proceedings of the National Academy of Sciences of the United States of America·Tung-Ching HoDing-Shinn Chen

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Methods Mentioned

BETA
laser capture microdissection
FACS
RNA-seq
PCA
biopsies
PCR

Software Mentioned

FlowJo
Cufflinks
FastQC
IPA ( Inguinity Pathway Analysis )
Cuffdiff
Genepattern
RStudio
Photoshop
Bioconductor
IPA

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