RNA-Sequencing-Based Transcriptomic Analysis Reveals a Role for Annexin-A1 in Classical and Influenza A Virus-Induced Autophagy.

Cells
Jianzhou CuiLina H K Lim

Abstract

Influenza viruses have been shown to use autophagy for their survival. However, the proteins and mechanisms involved in the autophagic process triggered by the influenza virus are unclear. Annexin-A1 (ANXA1) is an immunomodulatory protein involved in the regulation of the immune response and Influenza A virus (IAV) replication. In this study, using clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 (CRISPR associated protein 9) deletion of ANXA1, combined with the next-generation sequencing, we systematically analyzed the critical role of ANXA1 in IAV infection as well as the detailed processes governing IAV infection, such as macroautophagy. A number of differentially expressed genes were uniquely expressed in influenza A virus-infected A549 parental cells and A549 ∆ANXA1 cells, which were enriched in the immune system and infection-related pathways. Gene ontology and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway revealed the role of ANXA1 in autophagy. To validate this, the effect of mechanistic target of rapamycin (mTOR) inhibitors, starvation and influenza infection on autophagy was determined, and our results demonstrate that ANXA1 enhances autophagy induced by conventional autophagy induc...Continue Reading

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Citations

Nov 19, 2020·Cells·Ursula RescherJyoti K Jaiswal
Aug 25, 2021·Bioorganic Chemistry·Weiping LyuHaopeng Sun

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Datasets Mentioned

BETA
PRJNA637807

Methods Mentioned

BETA
transfection
chips
Assay
RNA-Seq
electrophoresis

Software Mentioned

Bioconductor package EdgeR
Sequence Read Archive
HTSeq
EdgeR
GSEA
WebGestalt
Spliced Transcripts Alignment to a Reference ( STAR ) aligner
DAVID
HTSEQ )
COSMID

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