RNA sequencing data for gamma radiation response in the extremotolerant tardigrade Ramazzottius varieornatus.

Data in Brief
Yuki YoshidaKazuharu Arakawa

Abstract

Tardigrades are microscopic animals of which terrestrial species are capable of tolerating extreme environments by entering a desiccated ametabolic state known as anhydrobiosis. Intriguingly, they survive high dosage gamma rays (>4,000 Gy), possibly through a mechanism known as cross-tolerance. We hypothesized that anhydrobiosis genes are also regulated during cross-tolerance, thus we submitted Ramazzottius varieornatus to 500 Gy 60Co gamma-ray and conducted time-course low-input RNA-Seq. The gene expression was quantified with RSEM and differential expression was determined with DEseq2. Differentially expressed genes were submitted to gene ontology enrichment analysis with GOStat. The transcriptome dynamically shifted nine hours post-exposure.

References

Nov 14, 2006·Bioinformatics·S Falcon, R Gentleman
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders

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Software Mentioned

ggplot2
bcl2fastq
bowtie2
bwa MEM
language Genome Analysis Environment
GOstat
RSEM
Trinity suite
DESeq2

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