RNA sequencing reveals retinal transcriptome changes in STZ-induced diabetic rats

Molecular Medicine Reports
Yuan-Jie LiuHui-Jun Yang

Abstract

The present study aimed to investigate changes in retinal gene expression in streptozotocin (STZ)‑induced diabetic rats using next‑generation sequencing, utilize transcriptome signatures to investigate the molecular mechanisms of diabetic retinopathy (DR), and identify novel strategies for the treatment of DR. Diabetes was chemically induced in 10‑week‑old male Sprague‑Dawley rats using STZ. Flash‑electroretinography (F‑ERG) was performed to evaluate the visual function of the rats. The retinas of the rats were removed to perform high throughput RNA sequence (RNA‑seq) analysis. The a‑wave, b‑wave, oscillatory potential 1 (OP1), OP2 and ∑OP amplitudes were significantly reduced in the diabetic group, compared with those of the control group (P<0.05). Furthermore, the implicit b‑wave duration 16 weeks post‑STZ induction were significantly longer in the diabetic rats, compared with the control rats (P<0.001). A total of 868 genes were identified, of which 565 were upregulated and 303 were downregulated. Among the differentially expressed genes (DEGs), 94 apoptotic genes and apoptosis regulatory genes, and 19 inflammatory genes were detected. The results of the KEGG pathway significant enrichment analysis revealed enrichment in cel...Continue Reading

References

Jan 1, 1986·Current Topics in Developmental Biology·R M ClaytonL H Errington
Jan 1, 1985·Vision Research·H HeynenD van Norren
Mar 1, 1996·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·J G Robson, L J Frishman
Mar 1, 1996·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·D C Hood, D G Birch
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 27, 2002·The British Journal of Ophthalmology·A P Adamis
Dec 12, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Antonia M JoussenLloyd Paul Aiello
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Irene NunesStephen P Goff
Apr 11, 2003·Diabetologia·B V BuiA J Vingrys
Jun 28, 2003·Brain Research. Molecular Brain Research·Dong-Youn HwangKwang-Soo Kim
Feb 27, 2004·Investigative Ophthalmology & Visual Science·Heather A Hancock, Timothy W Kraft
Aug 25, 2004·Investigative Ophthalmology & Visual Science·Pamela M MartinSylvia B Smith
Oct 1, 2004·Documenta Ophthalmologica. Advances in Ophthalmology·Michael F MarmorUNKNOWN International Society for Clinical Electrophysiology of Vision
Nov 24, 2004·Investigative Ophthalmology & Visual Science·Joanna A PhippsAlgis J Vingrys
Jun 27, 2006·Investigative Ophthalmology & Visual Science·Joanna A PhippsAlgis J Vingrys
Jul 18, 2006·Nucleic Acids Research·Jia YeJun Wang
Oct 27, 2006·Investigative Ophthalmology & Visual Science·David J RamseyHaohua Qian
Dec 15, 2006·Progress in Retinal and Eye Research·Usha P Andley
Apr 5, 2007·BMC Bioinformatics·Fátima Al-ShahrourJoaquín Dopazo
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Dana T Graves, Rayyan A Kayal
May 20, 2008·Nucleic Acids Research·Qi Zheng, Xiu-Jie Wang
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Sep 4, 2008·Journal of Periodontology·George L King
Jan 28, 2009·Pharmacotherapy·Tayyeba K Ali, Azza B El-Remessy
Mar 12, 2010·Nature·Stephen B MontgomeryEmmanouil T Dermitzakis
Aug 19, 2010·The Journal of Biological Chemistry·Judith Storch, Alfred E Thumser
Apr 7, 2011·Investigative Ophthalmology & Visual Science·Mandy K Losiewicz, Patrice E Fort
May 11, 2011·Immunotherapy·Wenbo ZhangRuth B Caldwell
Jun 4, 2011·Progress in Retinal and Eye Research·Johnny Tang, Timothy S Kern
Jul 19, 2011·Neuroscience Letters·Ji-Hong YangZheng Guo
Nov 1, 2011·Documenta Ophthalmologica. Advances in Ophthalmology·Donald C HoodUNKNOWN International Society For Clinical Electrophysiology of Vision
Mar 20, 2012·Circulation Research·Nikolina Papac-MilicevicBernd R Binder
Aug 4, 2012·ACS Chemical Neuroscience·Ellen GrünewaldPippa A Thomson
Aug 14, 2012·The British Journal of Ophthalmology·Rafael SimóUNKNOWN European Consortium for the Early Treatment of Diabetic Retinopathy (EUROCONDOR)
Jun 22, 2013·Nature Reviews. Immunology·Theresa L MurphyKenneth M Murphy
Dec 7, 2013·The International Journal of Developmental Biology·Nafees AhmadJochen Graw

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Methods Mentioned

BETA
RNA-seq
PCR

Software Mentioned

Statistical Package for the Social Sciences
WEGO
Blast2GO

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