PMID: 7518923Jul 19, 1994Paper

RNA template-directed RNA synthesis by T7 RNA polymerase

Proceedings of the National Academy of Sciences of the United States of America
C Cazenave, O C Uhlenbeck

Abstract

In an attempt to synthesize an oligoribonucleotide by run-off transcription by bacteriophage T7 RNA polymerase, a major transcript was produced that was much longer than expected. Analysis of the reaction indicated that the product resulted from initial DNA-directed run-off transcription followed by RNA template-directed RNA synthesis. This reaction occurred because the RNA made from the DNA template displayed self-complementarity at its 3' end and therefore could form an intra- or intermolecular primed template. In reactions containing only an RNA template, the rate of incorporation of NTPs was quite comparable to DNA-dependent transcription. RNA template-directed RNA synthesis has been found to occur with a great number of oligoribonucleotides, even with primed templates that are only marginally stable. In one instance, we observed a multistep extension reaction converting the oligonucleotide into a final product longer than twice its original length. Presumably, such a process could have generated some of the RNAs found to be efficiently replicated by T7 RNA polymerase.

References

May 30, 1978·Biochemistry·T E England, O C Uhlenbeck
May 1, 1990·Electrophoresis·R F JohnstonD L Barker
Aug 25, 1987·Nucleic Acids Research·L Sharmeen, J Taylor
Nov 11, 1987·Nucleic Acids Research·J F MilliganO C Uhlenbeck

❮ Previous
Next ❯

Citations

Jul 31, 2013·Immunobiology·Martin Schlee
Mar 25, 1998·FEBS Letters·E E RusakovaS N Kochetkov
Jul 10, 2003·Journal of Molecular Biology·C T Ranjith-KumarC Cheng Kao
Mar 26, 2003·European Journal of Biochemistry·Genoveva A Nacheva, Alfredo Berzal-Herranz
Jul 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Andreas SchmidtSimon Rothenfusser
Jul 22, 1998·Nucleic Acids Research·N Arnaud-BarbeF Mallet
Jun 19, 2001·Nucleic Acids Research·D Loakes
Oct 25, 2003·Nucleic Acids Research·Stacy L GelhausDaniele Fabris
Feb 20, 2004·Nucleic Acids Research·Agnieszka WichlaczJerzy Ciesiołka
Nov 27, 2004·Journal of Virology·Meike HassStephan Günther
Sep 24, 2013·BMC Molecular Biology·Katherine C Goldfarb, Thomas R Cech
Nov 3, 2012·RNA·Daniel KolakofskyStephen Cusack
Sep 14, 2010·RNA·Krishnamurthy MalathiRobert H Silverman
Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Amy M Williams, Susan A Martinis
Apr 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jean GuillerezMarc Dreyfus
Apr 19, 2011·Trends in Biochemical Sciences·J Bradford BowzardSuryaprakash Sambhara
Feb 14, 2015·Virus Research·Marian J KillipRichard E Randall
Jan 6, 2009·Immunological Reviews·Martin SchleeGunther Hartmann
Mar 10, 2015·Virology·Alison M Kell, Michael Gale
Feb 3, 2007·Journal of Molecular Biology·Mir Hussain NawazSusan A Martinis
Oct 4, 2016·Methods : a Companion to Methods in Enzymology·Jonathan JagodnikMaude Guillier
Dec 1, 2009·Viruses·Bianca Dauber, Thorsten Wolff
Mar 28, 1997·The Journal of Biological Chemistry·S S Sastry, B M Ross
Nov 13, 2001·The Journal of Biological Chemistry·Kathleen E McGinness, Gerald F Joyce
Apr 20, 2010·The Journal of Biological Chemistry·Jean-Baptiste MarqDominique Garcin
Oct 27, 2018·RNA·Jessica Döring, Thomas Hurek
Dec 16, 2006·Molecular Systems Biology·Jongmin KimErik Winfree
Mar 27, 2019·Future Medicinal Chemistry·Zhen Huang, Li Niu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.